RT Journal Article
T1 Geminivirus Replication Protein impairs SUMO conjugation of PCNA at two acceptor sites.
A1 Arroyo-Mateos, Manuel
A1 Sabarit Penalosa, Blanca
A1 Maio, Francesca
A1 Sánchez-Durán, Miguel A.
A1 Rosas-Díaz, Tábata Victoria
A1 Prins, Marcel
A1 Ruiz-Albert, Francisco Javier
A1 Pérez-Luna, Ana Isabel
A1 Van den Burg, Harrold
A1 Rodríguez-Bejarano, Eduardo
K1 Virus fitopatógenos - Genética
AB Geminiviruses are DNA viruses that replicate in nuclei of infected plant cells using the plant DNA replication machinery, including PCNA (proliferating cellular nuclear antigen), a cofactor that orchestrates genome duplication and maintenance by recruiting crucial players to replication forks. These viruses encode a multifunctional protein, Rep, which is essential for viral replication, induces the accumulation of the host replication machinery, and interacts with several host proteins, including PCNA and the SUMO E2 conjugation enzyme (SCE1). Posttranslational modification of PCNA by ubiquitin or SUMO plays an essential role in the switching of PCNA between interacting partners during DNA metabolism processes (e.g., replication, recombination, and repair, etc.). In yeast, PCNA sumoylation has been associated with DNA repair involving homologous recombination (HR). Previously, we reported that ectopic Rep expression results in very specific changes in the sumoylation pattern of plant cells. In this work, we show, using a reconstituted sumoylation system in Escherichia coli, that tomato PCNA is sumoylated at two residues, K254 and K164, and that coexpression of the geminivirus protein Rep suppresses sumoylation at these lysines. Finally, we confirm that PCNA is sumoylated in planta and that Rep also interferes with PCNA sumoylation in plant cells
PB ASM Journals
YR 2018
FD 2018-06-27
LK https://hdl.handle.net/10630/29952
UL https://hdl.handle.net/10630/29952
LA eng
NO Arroyo-Mateos M, Sabarit B, Maio F, Sánchez-Durán MA, Rosas-Díaz T, Prins M, RuizAlbert J, Luna AP, van den Burg HA, Bejarano ER. 2018. Geminivirus replication protein impairs SUMO conjugation of proliferating cellular nuclear antigen at two acceptor sites. J Virol 92:e00611-18. https://doi.org/10.1128/JVI .00611-18.
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DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026