RT Journal Article
T1 In Vivo Bioluminescence Imaging of Cell Differentiation in Biomaterials: A Platform for Scaffold Development.
A1 Bagó, Juli R
A1 Aguilar, Elisabeth
A1 Alieva, María
A1 Soler-Botija, Carolina
A1 Vila, Olaia F
A1 Claros-Gil, Silvia
A1 Andrades-Gómez, José Antonio
A1 Becerra-Ratia, José
A1 Rubio, Nuria
A1 Blanco, Jerónimo
K1 Bioluminiscencia
K1 Materiales biomédicos
K1 Células - Diferenciación
AB In vivo testing is a mandatory last step in scaffold development. Agile longitudinal noninvasive real-time monitoring of stem cell behavior in biomaterials implanted in live animals should facilitate the development of scaffolds for tissue engineering. We report on a noninvasive bioluminescence imaging (BLI) procedure for simultaneous monitoring of changes in the expression of multiple genes to evaluate scaffold performance in vivo. Adipose tissue-derived stromal mensenchymal cells were dually labeled with Renilla red fluorescent protein and firefly green fluorescent protein chimeric reporters regulated by cytomegalovirus and tissue-specific promoters, respectively. Labeled cells were induced to differentiate in vitro and in vivo, by seeding in demineralized bone matrices (DBMs) and monitored by BLI. Imaging results were validated by RT-polymerase chain reaction and histological procedures. The proposed approach improves molecular imaging and measurement of changes in gene expression of cells implanted in live animals. This procedure, applicable to the simultaneous analysis of multiple genes from cells seeded in DBMs, should facilitate engineering of scaffolds for tissue repair.
PB Mary Ann Liebert
YR 2012
FD 2012-12-19
LK https://hdl.handle.net/10630/33769
UL https://hdl.handle.net/10630/33769
LA eng
NO In Vivo Bioluminescence Imaging of Cell Differentiation in Biomaterials: A Platform for Scaffold Development Juli R. Bagó, Elisabeth Aguilar, Maria Alieva, Carolina Soler-Botija, Olaia F. Vila, Silvia Claros, José A. Andrades, José Becerra, Nuria Rubio, and Jerónimo Blanco Tissue Engineering Part A 2013 19:5-6, 593-603
NO https://v2.sherpa.ac.uk/id/publication/41517?template=romeo
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026