RT Journal Article
T1 The treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect
A1 Tamimi-Mariño, Iskandar Ahmad
A1 Gasca, María
A1 Halbardier, Alexandra
A1 Martin, Sergio
A1 Martin Caballero, Gregorio
A1 Lucena Serrano, Cristina
A1 Martin, Elena
A1 Tamimi, Faleh
A1 González-Quevedo, David
A1 García-de-Quevedo-Puerta, David
A1 Sobrino, Beatriz
A1 Palop, Begoña
A1 Guerado-Parra, Enrique
A1 Pérez Lara, Almudena Ester
A1 Urdiales, Cristina
K1 Infecciones quirúrgicas
K1 Artroplastia
AB Introduction: The formation of bacterial biofilms on knee arthroplasty implants can have catastrophic consequences. The aim of this study was to analyze the effectiveness of the bioelectric effect in the elimination of bacterial biofilms on cultivated knee arthroplasty implants. Methods: A novel device was designed to deliver a bioelectric effect on the surface of knee arthroplasty implants. 4-femoral prosthetic implants were cultivated with a staphylococcus aureus inoculum for 15 days. The components were divided into four different groups: A (not treated), B (normal saline 20-minutes), C (bioelectric effect 10-minutes), D (bioelectric effect 20-minutes). The implants were sonicated, and the detached colonies were quantified as the number of colony-forming unit (CFUs). The implants were sterilised and the process was repeated in a standardized manner four more times, to obtain a total of five samples per group. Results: The number of the CFUs after a 10-minute exposure to the bioelectric effect was of 208.2 ± 240.4, compared with 6,041.6 ± 2010.7 CFUs in group A, representing a decrease of 96.5% ± 4.3 (p = 0.004). And a diminution of 91.8% ± 7.9 compared with 2,051.0 ± 1,364.0 CFUs in group B (p = 0.109). The number of bacterial colonies after a 20-minute exposure to the bioelectric effect was 70 ± 126.7 CFUs, representing a decrease of 98.9% ± 1.9 (p = 0.000) compared with group A. And a decrease of 97.8% ± 3.0 (p = 0.019) compared with group B. Conclusions: The bioelectric effect was effective in the elimination of bacterial biofilm from knee arthroplasty implants. This method could be used in the future as part of conventional surgical procedures.
PB Frontiers
YR 2024
FD 2024-07-02
LK https://hdl.handle.net/10630/35669
UL https://hdl.handle.net/10630/35669
LA spa
NO Tamimi I, Gasca M, Halbardier A, et al. The treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect. Front Bioeng Biotechnol. 2024;12:1426388. Published 2024 Jul 2. doi:10.3389/fbioe.2024.1426388
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026