RT Conference Proceedings
T1 Comparative Analysis of the Type III Secretion System Effector Repertoires of Pseudomonas savastanoi Pathovars Pathogenic on Woody Hosts
A1 Moreno-Pérez, Alba
A1 Castañeda-Ojeda, M. Pilar
A1 Murillo, Jesús
A1 López-Solanilla, Emilia
A1 Rodríguez-Palenzuela, Pablo
A1 Ramos-Rodríguez, Cayo Juan
K1 Pseudomonas
AB The species Pseudomonas savastanoi, a member of the Pseudomonas syringae complex,includes four pathovars causing knots or excrescences in woody hosts: P. savastanoi pv.savastanoi (Psv), pv. fraxini (Psf), pv. nerii (Psn) and pv. retacarpa (Psr), comprising isolatesfrom olive, ash, oleander and broom plants, respectively. Pathogenicity of P. savastanoi isdependent, among other factors, on the type III secretion system (T3SS) and its effector(T3E) repertoire. Furthermore, a putative role in the interaction with woody hosts has beensuggested for several of these T3E. The recent availability of the genome sequences ofseveral P. savastanoi strains isolated from different hosts has facilitated bioinformaticspredictions of their T3SS genes and T3E pools, the study of their distribution in other strainsof the P. syringae complex isolated from woody hosts and the functional analysis of severalof these secreted proteins. As previously reported for Psv, Psn and Psf, here we show thatpathogenicity of Psr ICMP16945, is also dependent on the T3SS. Psv strains NCPPB 3335,ICMP4352 and PseNe107 share a core set of at least 22 T3E, 18 of which are also encoded inPsn ICMP16943, Psf ICMP7711 and Psr ICMP16945. However, these three strains encodetruncated versions of 1-2 of these 18 T3E and, Psr ICMP16945 contains three pathovarspecificT3E. Our results also show that several T3E, including HopAO1, are phylogeneticallyclustered across the P. syringae complex according to the woody/herbaceous nature of theirhost of isolation, suggesting host specialization of these effectors in this complex.
YR 2017
FD 2017-09-26
LK http://hdl.handle.net/10630/14535
UL http://hdl.handle.net/10630/14535
LA eng
NO Comunicación de tipo póster
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026