RT Journal Article
T1 Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture
A1 García-Márquez, Jorge
A1 Domínguez-Maqueda, Marta
A1 Pérez-Gómez, Olivia
A1 Cerezo, Isabel M.
A1 Espinosa Ruíz, Cristóbal
A1 Esteban, M. Ángeles
A1 Vallejo, Fernando
A1 Alarcón López, Francisco Javier
A1 Martínez Manzanares, Eduardo
A1 Tapia-Paniagua, Silvana Teresa
A1 Balebona-Accino, María del Carmen
A1 Moriñigo-Gutiérrez, Miguel Ángel
A1 Arijo-Andrade, Salvador
K1 Bacterias gram negativas
K1 Acuicultura
AB Vibrio proteolyticus DCF12.2 has demonstrated its ability to be used as a probiotic for fish species. This study investigates how different culture conditions influence the activity of its extracellular products (ECPs) in aquaculture, focusing on enzymatic and antibacterial activity, cytotoxicity, biofilm modulation, short-chain fatty acid (SCFA) profiles, and effects on Photobacterium damselae subsp. piscicida virulence. Enzymatic assays showed a variety of hydrolytic activities, including amylase, caseinase, and collagenase, which can enhance digestion and nutrient absorption in fish. Antibacterial assays revealed that ECPs from V. proteolyticus grown in an experimental aquafeed and a partial replacement of that aquafeed by 25% of a blend of microalgae inhibited P. damselae subsp. piscicida and P. damselae subsp. damselae. Cytotoxicity assays indicated variable effects across fish cell lines, with increased viability in SAF-1 and DLB-1 cells under specific conditions, and decreased viability in PLHC-1 cells, suggesting potential antitumor properties. Biofilm assays showed that certain ECP conditions reduced biofilm formation by Vibrio anguillarum, Aeromonas hydrophila, and Tenacibaculum maritimum. SCFA profiling detected acetic, iso-valeric, butyric, and valeric acids, which may contribute to antimicrobial activity and gut health. The ECPs significantly downregulated aip56 gene transcription, reducing the virulence of P. damselae subsp. piscicida. These findings suggest that ECPs from V. proteolyticus could be valuable aquafeed additives for enhancing fish nutrition, health, and disease resistance. Future research should aim to isolate and characterize the specific bioactive compounds responsible for these effects and elucidate their mechanisms of action for optimized application in aquaculture and other biotechnological fields.
PB Springer Nature
YR 2025
FD 2025-08-02
LK https://hdl.handle.net/10630/39628
UL https://hdl.handle.net/10630/39628
LA eng
NO Jorge García‑Márquez, Marta Domínguez‑Maqueda, Olivia Pérez‑Gómez, Isabel M. Cerezo, Cristóbal Espinosa‑Ruíz, M. Ángeles Esteban, Fernando Vallejo, Francisco Javier Alarcón‑López, Eduardo Martínez‑Manzanares, Silvana Teresa Tapia‑Paniagua, María Carmen Balebona, Miguel Ángel Moriñigo, Salvador Arijo (2025). Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture. Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture. Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture. Marine Biotechnology 27: 120. https://doi.org/10.1007/s10126-025-10500-6
NO Funding for open access charge: Universidad de Málaga/CBUA.
NO Ministerio de Ciencia e Innovación de España
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026