RT Journal Article
T1 Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA.
A1 Carrión Bravo, Víctor José
A1 Van der Voort, Menno
A1 Arrebola-Díez, Eva María
A1 Gutiérrez Barranquero, José Antonio
A1 De-Vicente-Moreno, Antonio
A1 Raaijmakers, Jos M.
A1 Cazorla-López, Francisco Manuel
K1 Fitotoxinas
K1 Antimetabolitos
K1 Bacterias fitopatógenas
K1 Operones
AB Background: The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringaestrains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis isgoverned by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affectmangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD.Results: The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoAresulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses byqPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes.Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under thenative promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, butnot in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegensPf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter.Conclusions: From the data obtained in this study, we conclude that both mbo and mgo operons are under thecontrol of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator ofmangotoxin biosynthesis.
PB BMC
YR 2014
FD 2014-02-21
LK https://hdl.handle.net/10630/31134
UL https://hdl.handle.net/10630/31134
LA eng
NO Carrión VJ, van der Voort M, Arrebola E, Gutiérrez-Barranquero JA, de Vicente A, Raaijmakers JM, Cazorla FM. Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA. BMC Microbiol. 2014 Feb 21;14:46.
NO This work was supported by grants from the Regional Government of Andalucía (Spain), grants from CICE - Junta de Andalucía, Ayudas Grupo PAIDI AGR-169, and Proyecto de Excelencia (P07-AGR-02471) and Plan Nacional de I + D + I del Ministerio de Ciencia e Innovacion (AGL2011-30354-C02-01) cofinanced by FEDER (EU). Plan Propio of the University of Málaga funded a stay of VJC in the Wageningen University, The Netherlands.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026