RT Journal Article
T1 iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migration Stimulation.
A1 López-Caraballo, Lidia
A1 Martorell-Marugán, Jordi
A1 Carmona-Sáez, Pedro
A1 González-Muñoz, María Elena
K1 Esclerosis múltiple
K1 Medicina - Modelos matemáticos
K1 Células madre embrionarias
K1 Proteómica
AB The most challenging aspect of secondary progressive multiple sclerosis (SPMS) is the lack of efficient regenerative response for remyelination, which is carried out by the endogenous population of adult oligoprogenitor cells (OPCs) after proper activation. OPCs must proliferate and migrate to the lesion and then differentiate into mature oligodendrocytes. To investigate the OPC cellular component in SPMS, we developed induced pluripotent stem cells (iPSCs) from SPMS-affected donors and age-matched controls (CT). We confirmed their efficient and similar OPC differentiation capacity, although we reported SPMS-OPCs were transcriptionally distinguishable from their CT counterparts. Analysis of OPC-generated conditioned media (CM) also evinced differences in protein secretion. We further confirmed SPMS-OPC CM presented a deficient capacity to stimulate OPC in vitro migration that can be compensated by exogenous addition of specific components. Our results provide an SPMS-OPC cellular model and encouraging venues to study potential cell communication deficiencies in the progressive form of multiple sclerosis (MS) for future treatment strategies.
PB MDPI
YR 2020
FD 2020-07-29
LK https://hdl.handle.net/10630/38822
UL https://hdl.handle.net/10630/38822
LA eng
NO Lopez-Caraballo, L., Martorell-Marugan, J., Carmona-Sáez, P., & Gonzalez-Munoz, E. (2020). iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migration Stimulation. Cells, 9(8), 1803. https://doi.org/10.3390/cells9081803
NO MINECO-SAF2015-66105-R
NO RyC 2014-15410
NO UMA18-FEDERJA-107
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026