RT Journal Article
T1 Identification of Host Genes Involved in Geminivirus Infection Using a Reverse Genetics Approach
A1 Lozano Durán, Rosa
A1 Rosas-Díaz, Tábata Victoria
A1 Pérez-Luna, Ana Isabel
A1 Rodríguez-Bejarano, Eduardo
K1 Virus
AB Geminiviruses, like all viruses, rely on the host cell machinery to establish a successful infection, but the identity andfunction of these required host proteins remain largely unknown. Tomato yellow leaf curl Sardinia virus (TYLCSV), amonopartite geminivirus, is one of the causal agents of the devastating Tomato yellow leaf curl disease (TYLCD). Thetransgenic 2IRGFP N. benthamiana plants, used in combination with Virus Induced Gene Silencing (VIGS), entail animportant potential as a tool in reverse genetics studies to identify host factors involved in TYLCSV infection. Using thesetransgenic plants, we have made an accurate description of the evolution of TYLCSV replication in the host in both spaceand time. Moreover, we have determined that TYLCSV and Tobacco rattle virus (TRV) do not dramatically influence eachother when co-infected in N. benthamiana, what makes the use of TRV-induced gene silencing in combination with TYLCSVfor reverse genetic studies feasible. Finally, we have tested the effect of silencing candidate host genes on TYLCSV infection,identifying eighteen genes potentially involved in this process, fifteen of which had never been implicated in geminiviralinfections before. Seven of the analyzed genes have a potential anti-viral effect, whereas the expression of the other elevenis required for a full infection. Interestingly, almost half of the genes altering TYLCSV infection play a role in postranslationalmodifications. Therefore, our results provide new insights into the molecular mechanisms underlying geminivirus infections,and at the same time reveal the 2IRGFP/VIGS system as a powerful tool for functional reverse genetics studies.
PB PlosONE
YR 2011
FD 2011-07-26
LK https://hdl.handle.net/10630/33410
UL https://hdl.handle.net/10630/33410
LA spa
NO Lozano-Durán R, Rosas-Díaz T, Luna AP, Bejarano ER (2011) Identification of Host Genes Involved in Geminivirus Infection Using a Reverse Genetics Approach. PLoS ONE 6(7): e22383. https://doi.org/10.1371/journal.pone.0022383
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026