RT Generic
T1 Dataset Orbitrap Raw-Data Stress Hypothalamus(1h-24h)
A1 Infantes-López, M. Inmaculada
A1 Nieto-Quero, Andrea
A1 Pérez-Martín, Margarita
A1 Pedraza-Benítez, María del Carmen
K1 Estrés (Psicología)
K1 Hipotálamo
AB We have examined the effect of an acute stress in the proteomic profile of hypothalamus. For this purpose, to extract information from proteomic profiles (1 and 24 hours post-acute stress) a Quadrupole-Orbitrap mass spectrometry (Q-Orbitrap-MS) was used for the massive identification and quantification of hypothalamic proteins. After collecting the raw data extracted from this technique, for each proteomic profile, a functional enrichment study was performed. Firstly, the significantly overexpressed and underexpressed hypothalamic proteins of the right hemisphere of experimental groups with their respective controls (stress/control) were selected. Then, using STRING, the number of interactors was increased to 100 for both over- and under-expressed proteins. Furthermore, MCODE was used as a clustering tool for the protein‒protein interaction network obtained from STRING. After that, a functional enrichment analysis was performed using DAVID by introducing each protein gene name grouped into the clusters from the previous step. Biological processes in each protein clustering were determined as well as the function it is involved in. Finally, a network analysis of the GO terms obtained from DAVID was performed so that it was easier to interpret and integrate the biological implications of the results. Data were validate by using a western blot analysis. In the end, the main conclusion was that this short-term stressor altered the protein profile of the hypothalamus, changing the protein abundance after 1 hour or 24 hours of stress application.
PB Universidad de Málaga
YR 2023
FD 2023-03-30
LK https://hdl.handle.net/10630/26229
UL https://hdl.handle.net/10630/26229
LA eng
NO - Ministerio de Ciencia e Innovación – Plan Nacional I+D+I from Spain (PID2020-117464RB-I00) to CP and MP-M; - FEDER/Ministerio de Ciencia e Innovación – Agencia Estatal de Investigación Investigación from Spain (PSI2017-83408-P) to CP- FEDER/Junta de Andalucía – Proyectos I+D+I en el marco del Programa Operativo FEDER Andalucía 2014-2020 (UMA20-FEDERJA-112) to CP and MP-M- Consejería de Conocimiento, Investigación y Universidades, Junta de Andalucía (P20_00460) to CP.- Ministerio de Universidades from Spain: FPU16/05308 to AN-Q and FPU19/03629 to MII-L.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026