RT Journal Article
T1 Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells.
A1 García-Bonilla, María
A1 Ojeda-Pérez, Betsaida
A1 García-Martín, María Luisa
A1 Muñoz-Hernández, María del Carmen
A1 Vitorica Ferrández, Javier
A1 Jiménez, Sebastián
A1 Cifuentes-Rueda, Manuel
A1 Santos-Ruiz, Leonor
A1 Shumilov, Kirill
A1 Claros-Gil, Silvia
A1 Gutiérrez-Pérez, Antonia
A1 Páez-González, Patricia
K1 Hidrocefalia
K1 Células madre
K1 Astrocitos
K1 Espectroscopía
AB In obstructive congenital hydrocephalus, cerebrospinal fluid accumulation is associated with high intracranial pressure and the presence of periventricular edema, ischemia/hypoxia, damage of the white matter, and glial reactions in the neocortex. The viability and short time effects of a therapy based on bone marrow-derived mesenchymal stem cells (BM-MSC) have been evaluated in such pathological conditions in the hyh mouse model. moBM-MSC expressing fluorescent mRFP1 protein were injected into the lateral ventricle of hydrocephalic hyh mice. The effect in the neocortex was compared with hydrocephalic hyh mice injected with the vehicle and non-hydrocephalic littermates. Neural cell populations and the possibility of transdifferentiation were analyzed. Tissue recovering was investigated by H-1 HR-MAS NMR spectroscopy, thus allowing the detection of metabolites/osmolytes related with hydrocephalus severity and outcome in the neocortex. An in vitro assay to simulate the periventricular astrocyte reaction conditions was performed using BM-MSC under high TNF alpha level condition. The secretome in the culture medium was analyzed in this assay. Four days after transplantation, BM-MSC were found undifferentiated and scattered into the astrocyte reaction present in the damaged neocortex white matter. Tissue rejection to the integrated BM-MSC was not detected 4 days after transplantation. Hyh mice transplanted with BM-MSC showed a reduction in the apoptosis in the periventricular neocortex walls, suggesting a neuroprotector effect of the BM-MSC in these conditions. A decrease in the levels of metabolites/osmolytes in the neocortex, such as taurine and neuroexcytotoxic glutamate, also indicated a tissue recovering. Under high TNF alpha level condition in vitro, BM-MSC showed an upregulation of cytokine and protein secretion that may explain homing, immunomodulation, and vascular permeability, and therefore the tissue recovering.
PB BMC
YR 2020
FD 2020-03-17
LK https://hdl.handle.net/10630/30530
UL https://hdl.handle.net/10630/30530
LA eng
NO García-Bonilla, M., Ojeda-Pérez, B., García-Martín, M.L. et al. Neocortical tissue recovery in severe congenital obstructive hydrocephalus after intraventricular administration of bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther 11, 121 (2020).
NO Instituto de Salud Carlos III (PI15/00619; PI19/00778; PI15/00796 y PI18/01557)Ministerio de Economía y Competitividad - MINECO (RYC-2014-16980)Ministerio de Educación, Cultura y Deporte - MECD (FPU13/02906)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026