RT Journal Article
T1 Epitranscriptome changes triggered by ammonium nutrition regulate the proteome response of maritime pine roots.
A1 Ortigosa Peña, Francisco
A1 Lobato-Fernández, César
A1 Pérez-Claros, Juan Antonio
A1 Ruiz-Canton, Francisco Javier
A1 Ávila-Sáez, Concepción
A1 Cánovas-Ramos, Francisco Miguel
A1 Cañas-Pendón, Rafael Antonio
K1 Genetica vegetal
K1 Proteínas - Metabolismo
K1 Amoniaco
K1 Pinos
AB Epitranscriptome constitutes a gene expression checkpoint in all living organisms. Nitrogen is an essential element for plant growth and development that influences gene expression at different levels such as epigenome, transcriptome, proteome, and metabolome. Therefore, our hypothesis is that changes in the epitranscriptome may regulate nitrogen metabolism. In this study, epitranscriptomic modifications caused by ammonium nutrition were monitored in maritime pine roots using Oxford Nanopore Technology. Transcriptomic responses mainly affected transcripts involved in nitrogen and carbon metabolism, defense, hormone synthesis/signaling, and translation. Global detection of epitranscriptomic marks was performed to evaluate this posttranscriptional mechanism in un/treated seedlings. Increased N6-methyladenosine (m6A) deposition in the 3’-UTR was observed in response to ammonium, which seems to be correlated with poly(A) lengths and changes in the relative abundance of the corresponding proteins. The results showed that m6A deposition and its dynamics seem to be important regulators of translation under ammonium nutrition. These findings suggest that protein translation is finely regulated through epitranscriptomic marks likely by changes in mRNA poly(A) length, transcript abundance and ribosome protein composition. An integration of multiomics data suggests that the epitranscriptome modulates responses to nutritional, developmental and environmental changes through buffering, filtering, and focusing the final products of gene expression.
PB Frontiers
SN ISSN:1664-462X
YR 2022
FD 2022
LK https://hdl.handle.net/10630/37936
UL https://hdl.handle.net/10630/37936
LA eng
NO Ortigosa F, Lobato-Ferna´ ndez C, Pe´rez-Claros JA, Canto´ n FR, A´ vila C, Ca´ novas FM and Cañas RA (2022) Epitranscriptome changes triggered by ammonium nutrition regulate the proteome response of maritime pine roots. Front. Plant Sci. 13:1102044. doi: 10.3389/fpls.2022.1102044
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026