RT Journal Article
T1 Inflammatory microglia are glycolytic and iron retentive and typify the microglia in APP/PS1 mice.
T2 The IFNg-induced shift to glycolysis in microglia is associated with changes in iron handling by cells.
A1 Holland, Roland
A1 McIntosh, Allison
A1 Finucane, Orla
A1 Mela-Rivas, Virginia
A1 Rubio-Araiz, Ana
A1 Timmons, G.
A1 McCarthy, S.
A1 Gunko, Y.K.
A1 Lynch, Marina Ann
K1 Microglia
K1 Glucólisis
K1 Hierro - Metabolismo
K1 Inflamación (Patología)
K1 Modelos animales en investigación
AB Microglia, like macrophages, can adopt inflammatory and anti-inflammatory phenotypesdepending on the stimulus. At the polar ends of the spectrum of phenotypes are the socalled M1-like inflammatory cells and M2-like anti-inflammatory cells that are stimulatedin vitro by lipopolysaccharide (LPS) or interferon- (IFN) and interleukin-4 (IL-4)respectively. In macrophages, the evidence indicates that these phenotypes have differentmetabolic profiles with M1-like cells switching to glycolysis as their main source of ATPand M2-like cells utilizing oxidative phosphorylation. There is a paucity of informationregarding the metabolic signatures in inflammatory and anti-inflammatory microglialphenotypes. Here, we polarized primary microglia with IFN and show that thecharacteristic increases in nitric oxide synthase 2 (NOS2) and tumor necrosis factor-α(TNF) were accompanied by increased glycolysis and an increase in the expression of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB)3, an enzyme that plays asignificant role in driving glycolysis. These changes were associated with increasedexpression of ferritin and retention of iron in microglia. In contrast with the IFN-inducedchanges IL-4, which predictably increased mRNA expression of mannose receptor C, type1 (MRC-1) and arginase 1 (Arg1), also increased oxygen consumption in microglia. Thedata indicate distinct metabolic signatures of inflammatory and anti-inflammatorymicroglia that are also distinguishable by their iron handling profiles.
PB Elsevier
YR 2018
FD 2018-02
LK https://hdl.handle.net/10630/40712
UL https://hdl.handle.net/10630/40712
LA eng
NO Holland R, McIntosh AL, Finucane OM, Mela V, Rubio-Araiz A, Timmons G, McCarthy SA, Gun'ko YK, Lynch MA. Inflammatory microglia are glycolytic and iron retentive and typify the microglia in APP/PS1 mice. Brain Behav Immun. 2018 Feb;68:183-196. doi: 10.1016/j.bbi.2017.10.017. Epub 2017 Oct 20. PMID: 29061364.
NO https://openpolicyfinder.jisc.ac.uk/id/publication/12478
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026