RT Generic
T1 Dataset for: Galanin(1− 15) and Naltrexone: A novel approach for alcohol use disorder in rats, involving the mesolimbic system.
A1 Flores Gómez, Marta
A1 Cantero-García, Noelia
A1 Pineda-Gómez, Juan Pedro
A1 Moh-Ahmed, Amel
A1 Flores-Burgess, Antonio
A1 Díaz-Cabiale, Zaida
A1 Millón-Peñuela, Carmelo
K1 Alcohol - Consumo
K1 Alcohol - Efectos fisiológicos
K1 Modelos animales en investigación
K1 Neuropéptidos
K1 Naltrexona
AB Alcohol Use Disorder (AUD) is a highly prevalent psychiatric and represents a significant public health challenge. Naltrexone (NTX), a mu-opioid receptor antagonist widely used for AUD treatment, has limited efficacy due to side effects and variability in patient response. Interactions between the full-length GAL molecule and the opioid system have been demonstrated. In our recent studies, we showed that the Galanin (1− 15) fragment [GAL (1− 15)] decreased alcohol seeking along with alcohol consumption. This study aims to examine the effects of GAL(1− 15)+NTX on alcohol-seeking behavior and alcohol consumption, as well as the involvement of the mesolimbic system. In rats, we assessed GAL(1− 15)+NTX in reward-seeking and the role of GALR2 using the antagonist M871 in the self-administration test. In addition, GAL(1− 15)+NTX effects were studied on voluntary alcohol using the two-bottle choice paradigm. Locomotor activity and stereotyped behaviors, along with dopa- mine release in the dorsal striatum following alcohol injections, were assessed. Moreover, we have analyzed the transcriptional changes of C-Fos, MOR, POMPC, and dopamine receptors in the ventral tegmental area, nucleus accumbens and the hypothalamus. GAL(1− 15)+NTX combination reduced alcohol seeking in self-administration and two-bottle choice consumption, with GALR2 involved in the effect. In addition, GAL(1− 15)+NTX attenuated alcohol-induced locomotor activity and stereotyped behaviors linked to reduced dopamine release in the dorsal striatum. Notably, these effects were associated with C-Fos, MOR, and dopamine receptor changes, suggesting that the mesolimbic pathway, including the opioid system, is involved in GAL(1− 15)+NTX effects. These results open up the possibility of using GAL(1− 15) with NTX as a novel strategy in AUD.
PB Universidad de Málaga
YR 2025
FD 2025
LK https://hdl.handle.net/10630/39860
UL https://hdl.handle.net/10630/39860
LA eng
NO This work was supported by grants awarded by Ministerio de Sanidad del gobierno de España, Delegación del Gobierno para el Plan Nacional sobre Drogas, Fondos de Recuperación, Transformación y Resiliencia (PRTR) Unión Europea EXP2022/008766, by Ministerio de Ciencia e Innovación del gobierno de España PID2020–114392RB-I00/AEI/10.13039/501100011033, and by Universidad de Málaga PPRO-B4–2024–010.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026