RT Journal Article
T1 Response of the Biocontrol Agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix Exudate
A1 Pliego, Clara
A1 Crespo-Gómez, José Ignacio
A1 Pintado Calvillo, Adrián
A1 Pérez-Martínez, Isabel
A1 De Vicente, Antonio
A1 Cazorla-López, Francisco Manuel
A1 Ramos-Rodríguez, Cayo Juan
K1 Agentes de control biológico de plagas
AB The rhizobacterium Pseudomonas pseudoalcaligenes AVO110, isolated bythe enrichment of competitive avocado root tip colonizers, controls avocado whiteroot rot disease caused by Rosellinia necatrix. Here, we applied signature-tagged mutagenesis (STM) during the growth and survival of AVO110 in fungal exudatecontaining medium with the goal of identifying the molecular mechanisms linked tothe interaction of this bacterium with R. necatrix. A total of 26 STM mutants outcompeted by the parental strain in fungal exudate, but not in rich medium, were selected and named growth-attenuated mutants (GAMs). Twenty-one genes wereidentified as being required for this bacterial-fungal interaction, including membranetransporters, transcriptional regulators, and genes related to the metabolism of hydrocarbons, amino acids, fatty acids, and aromatic compounds. The bacterial traitsidentified here that are involved in the colonization of fungal hyphae include proteins involved in membrane maintenance (a dynamin-like protein and ColS) orcyclic-di-GMP signaling and chemotaxis. In addition, genes encoding a DNA helicase(recB) and a regulator of alginate production (algQ) were identified as being requiredfor efficient colonization of the avocado rhizosphere.
PB American Society fo Microbilogy
YR 2019
FD 2019-01-23
LK https://hdl.handle.net/10630/40168
UL https://hdl.handle.net/10630/40168
LA eng
NO Pliego C, Crespo-Gómez JI, Pintado A, Pérez-Martínez I, de Vicente A, Cazorla FM, Ramos C. 2019. Response of the biocontrol agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix exudate. Appl Environ Microbiol 85:e01741-18. https://doi.org/10 .1128/AEM.01741-18
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026