RT Journal Article
T1 Involvement of autologous myeloid dendritic cells in the evaluation of immediate hypersensitivity reactions to betalactams
A1 Fernández-Santamaría, Rubén
A1 Ariza, Adriana
A1 Bogas, Gador
A1 Salas, Maria
A1 Calvo-Serrano, Silvia
A1 Frecha, Cecilia
A1 Mayorga Mayorga, Cristobalina
A1 Torres-Jaén, María Josefa
A1 Fernández-Duarte, Tahía Diana
K1 Antibióticos betalactámicos - Efectos secundarios
K1 Células dendríticas
K1 Alergia a los medicamentos
AB Background: Amoxicillin (AX) and clavulanic acid (CLV) are the betalactam antibiotics (BLs) most used to treat bacterial infections, although they can trigger immediate hypersensitivity reactions (IDHRs). The maturation analysis of monocyte-derived dendritic cells (moDCs) and their capacity to induce proliferative response of lymphocytes are useful to test the sensitisation to a drug, although without optimal sensitivity. Nevertheless, this can be improved using directly isolated DCs such as myeloid DCs (mDCs).Methods: mDCs and moDCs were obtained from 28 allergic patients (AP), 14 to AX, 14 to CLV and from 10 healthy controls (HC). The expression of CCR7, CD40, CD80, CD83, and CD86 was analysed after stimulation with both BLs. We measured the capacity of these pre-primed DCs to induce drug-specific activation of different lymphocyte subpopulations, CD3+, CD4+, CD8+, CD4+Th1, and CD4+Th2, by flow cytometry.Results: Higher expression of CCR7, CD40, CD80, CD83, and CD86 was observed on mDCs compared to moDCs from AP after stimulating with the culprit BL. Similarly, mDCs induced higher proliferative response, mainly of CD4+Th2 cells, compared to moDCs, reaching up to 67% of positive results with AX, whereas of only 25% with CLV.Conclusions: mDCs from selective AP efficiently recognise the culprit drug which trigger the IDHR. mDCs also trigger proliferation of lymphocytes, mainly those with a Th2 cytokine pattern, although these responses depend on the nature of the drug, mimicking the patient's reaction.
PB Elsevier
YR 2024
FD 2024
LK https://hdl.handle.net/10630/39757
UL https://hdl.handle.net/10630/39757
LA eng
NO Clinical immunology 2024;262:110166
NO RFS, AA, GB equally contributed to this work.  CM, MJT and TDF equally contributed to this work.
NO https://openpolicyfinder.jisc.ac.uk/id/publication/11277
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026