RT Journal Article
T1 A Molecularly Imprinted Polypyrrole/GO@Fe3O4 Nanocomposite Modified Impedimetric Sensor for the Routine Monitoring of Lysozyme
A1 Montoro-Leal, Pablo
A1 Morales Frías‬, ‪Isaac Aarón
A1 Vereda-Alonso, Elisa Isabel
A1 Errachid, Abdelhamid
A1 Jaffrezic-Renault, Nicole
K1 Lisozima
AB Lysozyme (LYS) applications encompass anti-bacterial activity, analgesic, and anti-inflammatory effects. In this work, a porous framework that was based on the polymerization of pyrrole (PPy) in the presence of multi-functional graphene oxide/iron oxide composite (GO@Fe3O4) has been developed. Oxygen-containing and amine groups that were present in the nanocomposite were availed to assembly LYS as the molecularly imprinted polymer (MIP) template. The synthesized material (MIPPy/GO@Fe3O4) was electrodeposited on top of a gold microelectrode array. Transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) were used to confirm the adequate preparation of GO@Fe3O4, and the characterization of the resulting molecularly imprinted electrochemical sensor (MIECS) was carried out by electrochemical impedance spectrometry (EIS), FT-IR analysis, and scanning electron microscopy (SEM). The impedimetric responses were analyzed mathematically by fitting to a Q(Q(RW)) equivalent circuit and quantitative determination of LYS was obtained in a linear range from 1 pg/mL to 0.1 µg/mL, presenting good precision (RSD ≈ 10%, n = 5) and low limit of detection (LOD = 0.009 pg/mL). The fabrication of this device is relatively simple, scalable, rapid, and economical, and the sensor can be used up to nine times without disintegration. The MIECS was successfully applied to the determination of LYS in fresh chicken egg white sample and in a commercial drug, resulting in a straightforward platform for the routine monitoring of LYS.
PB IOAP-MPDI
YR 2022
FD 2022-09-05
LK https://hdl.handle.net/10630/24999
UL https://hdl.handle.net/10630/24999
LA eng
NO Montoro-Leal P, Frías IAM, Vereda Alonso E, Errachid A, Jaffrezic-Renault N. A Molecularly Imprinted Polypyrrole/GO@Fe3O4 Nanocomposite Modified Impedimetric Sensor for the Routine Monitoring of Lysozyme. Biosensors. 2022; 12(9):727. https://doi.org/10.3390/bios12090727
NO N.J.-R.: Supervision, project administration, funding acquisition, writing—review and editing. A.E.: Supervision, project administration, funding acquisition, writing—review and editing. E.V.A.: Methodology, writing—review and editing. I.A.M.F.: Investigation, conceptualization, methodology, writing—original draft. P.M.-L.: Investigation, conceptualization, validation, writing—original draft. All authors have read and agreed to the published version of the manuscript.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026