RT Journal Article
T1 Graphene oxide and reduced derivates, as powder or film scaffolds, differentially promote dopaminergic neuron differentiation and survival.
A1 Rodríguez-Losada, Noela
A1 Wendelbo, Rune
A1 Ocaña, C
A1 Díaz-Casares, Amelia
A1 Guzmán de Villoria, Roberto
A1 Aguirre-Gómez, José Ángel
A1 Arráez-Sánchez, Miguel Ángel
A1 González-Alegre, P
A1 Medina, MA
A1 Arenas, Ernest
A1 Narváez-Bueno, José Ángel
K1 Grafeno
AB Emerging scaffold structures made of carbon nanomaterials, such as graphene oxide (GO) have shown efficient bioconjugation with common biomolecules. Previous studies described that GO promotes the differentiation of neural stem cells and may be useful for neural regeneration. In this study, we examined the capacity of GO, full reduced (FRGO), and partially reduced (PRGO) powder and film to support survival, proliferation, differentiation, maturation, and bioenergetic function of a dopaminergic (DA) cell line derived from the mouse substantia nigra (SN4741). Our results show that the morphology of the film and the species of graphene (GO, PRGO, or FRGO) influences the behavior and function of these neurons. In general, we found better biocompatibility of the film species than that of the powder. Analysis of cell viability and cytotoxicity showed good cell survival, a lack of cell death in all GO forms and its derivatives, a decreased proliferation, and increased differentiation over time. Neuronal maturation of SN4741 in all GO forms, and its derivatives were assessed by increased protein levels of tyrosine hydroxylase (TH), dopamine transporter (DAT), the glutamate inward rectifying potassium channel 2 (GIRK2), and of synaptic proteins, such as synaptobrevin and synaptophysin.
PB Frontiers
YR 2020
FD 2020
LK https://hdl.handle.net/10630/29654
UL https://hdl.handle.net/10630/29654
LA eng
NO Rodriguez-Losada N, Wendelbob R, Ocaña MC, Casares AD, Guzman de Villoría R, Aguirre Gomez JA, Arraez MA, Gonzalez-Alegre P, Medina MA, Arenas E and Narvaez JA (2020) Graphene Oxide and Reduced Derivatives, as Powder or Film Scaffolds, Differentially Promote Dopaminergic Neuron Differentiation and Survival. Front. Neurosci. 14:570409. doi: 10.3389/fnins.2020.570409
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026