RT Journal Article
T1 Artemia spp., a Susceptible Host and Vector for Lymphocystis Disease Virus.
A1 Jiménez Valverde, Estefanía
A1 Labella Vera, Alejandro Manuel
A1 Borrego-García, Juan José
A1 Castro-López, María Dolores
K1 Peces - Enfermedades por virus
AB Di erent developmental stages of Artemia spp. (metanauplii, juveniles and adults) werebath-challenged with two isolates of the Lymphocystis disease virus (LCDV), namely, LCDV SA25(belonging to the species Lymphocystis disease virus 3) and ATCC VR-342 (an unclassified memberof the genus Lymphocystivirus). Viral quantification and gene expression were analyzed by qPCRat di erent times post-inoculation (pi). In addition, infectious titres were determined at 8 dpi byintegrated cell culture (ICC)-RT-PCR, an assay that detects viral mRNA in inoculated cell cultures.In LCDV-challenged Artemia, the viral load increased by 2–3 orders of magnitude (depending ondevelopmental stage and viral isolate) during the first 8–12 dpi, with viral titres up to 2.3   102Most Probable Number of Infectious Units (MPNIU)/mg. Viral transcripts were detected in theinfected Artemia, relative expression values showed a similar temporal evolution in the di erentexperimental groups. Moreover, gilthead seabream (Sparus aurata) fingerlings were challenged byfeeding on LCDV-infected metanauplii. Although no Lymphocystis symptoms were observed in thefish, the number of viral DNA copies was significantly higher at the end of the experimental trial andmajor capsid protein (mcp) gene expression was consistently detected. The results obtained supportthat LCDV infects Artemia spp., establishing an asymptomatic productive infection at least under theexperimental conditions tested, and that the infected metanauplii are a vector for LCDV transmissionto gilthead seabream.
PB MDPI
YR 2019
FD 2019-06-01
LK https://hdl.handle.net/10630/34425
UL https://hdl.handle.net/10630/34425
LA eng
NO Valverde EJ, Labella AM, Borrego JJ, Castro D. Artemia spp., a Susceptible Host and Vector for Lymphocystis Disease Virus. Viruses. 2019 Jun 1;11(6):506. doi: 10.3390/v11060506. PMID: 31159450; PMCID: PMC6630821.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026