RT Conference Proceedings
T1 Root and shoot growth of Pinus strobus x P. wallichiana somatic plants is influenced by the nitrogen composition of the germination medium
A1 Llebrés, María Teresa
A1 Pascual-Moreno, María Belén
A1 Cánovas-Ramos, Francisco Miguel
A1 Ávila-Sáez, Concepción
A1 Klimaszewska K, 
K1 Pino - Embriogénesis somática
AB Somatic embryogenesis (SE) in conifers has proven to be an useful biotechnological tool forspecies conservation and mass propagation, but to make it commercially viable, adaptiveresearch is required to convert laboratory protocols into plantation reality. Hybrid white pinesare of interest to forest industry in North America due to the potential resistance to white pineblister rust (Cronartium ribicola). Somatic embryos of many pine species are typicallygerminated on the same nutrient medium as used for earlier stages of SE. For most of thegenotypes of white and hybrid white pine, the time required for the plantlets to grow a 2 cmroot takes approximately five to six months at which time the plantlets can be potted andtransferred to a greenhouse. The survival of plants is however sketchy and not always reliable.In order to accelerate and improve the root growth and survival of the plants in a greenhouse astudy was initiated on the manipulation of the nitrogen composition of the germinationmedium. We were interested in learning whether the pine somatic seedlings had a preferencefor organic or inorganic forms of N during the germination step and root growth. Experimentshave been conducted to determine the uptake and content of 15N in those roots by submergingthem in a hydroponic solution for two hours. The somatic seedlings’ root growth wasdramatically affected by the N composition of the medium as well as it did affect the uptake of15N. To follow up the nitrogen metabolism in somatic seedlings, we have performedmicroarray and qPCR analysis for nitrogen or nitrogen-related metabolism genes. The resultsof the analysis will be discussed.
YR 2013
FD 2013-11-07
LK http://hdl.handle.net/10630/6371
UL http://hdl.handle.net/10630/6371
LA spa
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026