RT Journal Article
T1 Characterization of adult stem/progenitor cell populations from bone marrow in a three-dimensional collagen gel culture system.
A1 Claros-Gil, Silvia
A1 Rodríguez-Losada, Noela
A1 Cruz, Encarnación
A1 Guerado-Parra, Enrique
A1 Becerra-Ratia, José
A1 Andrades-Gómez, José Antonio
K1 Células madre hematopoyéticas
K1 Huesos - Crecimiento
AB Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells that are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and nonadherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4, and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent-, hematopoietic-, mesenchymal-, and endothelial-specific markers in vitro and can undergo osteogenic differentiation in vivo.
PB Sage Publications
YR 2012
FD 2012-09-01
LK https://hdl.handle.net/10630/33561
UL https://hdl.handle.net/10630/33561
LA eng
NO Claros S, Rodríguez-Losada N, Cruz E, Guerado E, Becerra J, Andrades JA. Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System. Cell Transplantation. 2012;21(9):2021-2032.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026