RT Journal Article
T1 Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass
A1 Moreno García, Patricia
A1 Souto-Pereira, Sandra
A1 Leiva Rebollo, Rocío
A1 Bandín Matos, Isabel
A1 Alonso-Sánchez, María del Carmen
K1 Lubinas - Virus
AB European sea bass (Dicentrarchus labrax) is severely afected by nervous necrosis disease, caused bynervous necrosis virus (NNV). Two out of the four genotypes of this virus (red-spotted grouper nervousnecrosis virus, RGNNV; and striped jack nervous necrosis virus, SJNNV) have been detected in sea bass,although showing diferent levels of virulence to this fsh species. Thus, sea bass is highly susceptibleto RGNNV, whereas outbreaks caused by SJNNV have not been reported in this fsh species. The roleof the capsid protein (Cp) amino acids 247 and 270 in the virulence of a RGNNV isolate to sea bass hasbeen evaluated by the generation of recombinant RGNNV viruses harbouring SJNNV-type amino acidsin the above mentioned positions (Mut247Dl965, Mut270Dl965 and Mut247+270Dl965). Viral in vitroand in vivo replication, virus virulence and fsh immune response triggered by these viruses have beenanalysed. Mutated viruses replicated on E-11 cells, although showing some diferences compared to thewild type virus, suggesting that the mutations can afect the viral cell recognition and entry. In vivo, fshmortality caused by mutated viruses was 75% lower, and viral replication in sea bass brain was alteredcompared to non-mutated virus. Regarding sea bass immune response, mutated viruses triggered alower induction of IFN I system and infammatory response-related genes. Furthermore, mutationscaused changes in viral serological properties (especially the mutation in amino acid 270), inducinghigher seroconversion and changing antigen recognition.
PB Springer Nature
YR 2019
FD 2019-10-19
LK https://hdl.handle.net/10630/33092
UL https://hdl.handle.net/10630/33092
LA eng
NO Moreno, P., Souto, S., Leiva-Rebollo, R. et al. Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass. Sci Rep 9, 14068 (2019). https://doi.org/10.1038/s41598-019-50622-1
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026