RT Journal Article
T1 The NAC transcription factor FaRIF controls fruit ripening in strawberry.
A1 Martín-Pizarro, Carmen
A1 Vallarino, José G.
A1 Osorio-Algar, Sonia
A1 Meco, Victoriano
A1 Urrutia, María
A1 Pillet, Jeremy
A1 Casañal, Ana
A1 Merchante-Berg, Catharina
A1 Amaya, Iraida
A1 Willmitzer, Lothar
A1 Fernie, Alisdair R.
A1 Giovannoni, James J.
A1 Botella-Mesa, Miguel Ángel
A1 Valpuesta-Fernández, Victoriano
A1 Posé-Padilla, David
K1 Fresas - Maduración
K1 Genética vegetal
AB In contrast to climacteric fruits such as tomato, the knowledge on key regulatory genes controlling the ripening of strawberry, a nonclimacteric fruit, is still limited. NAC transcription factors (TFs) mediate different developmental processes in plants. Here, we identified and characterized Ripening Inducing Factor (FaRIF), a NAC TF that is highly expressed and induced in strawberry receptacles during ripening. Functional analyses based on stable transgenic lines aimed at silencing FaRIF by RNA interference, either from a constitutive promoter or the ripe receptacle-specific EXP2 promoter, as well as overexpression lines showed that FaRIF controls critical ripening-related processes such as fruit softening and pigment and sugar accumulation. Physiological, metabolome, and transcriptome analyses of receptacles of FaRIF-silenced and overexpression lines point to FaRIF as a key regulator of strawberry fruit ripening from early developmental stages, controlling abscisic acid biosynthesis and signaling, cell-wall degradation, and modification, the phenylpropanoid pathway, volatiles production, and the balance of the aerobic/anaerobic metabolism. FaRIF is therefore a target to be modified/edited to control the quality of strawberry fruits.
PB Oxford University Press [University Publisher], American Society of Plant Biologists [Society Publisher]
YR 2021
FD 2021-02-24
LK https://hdl.handle.net/10630/29630
UL https://hdl.handle.net/10630/29630
LA eng
NO Martín-Pizarro C, Vallarino JG, Osorio S, Meco V, Urrutia M, Pillet J, Casañal A, Merchante C, Amaya I, Willmitzer L, Fernie AR, Giovannoni JJ, Botella MA, Valpuesta V, Posé D. The NAC transcription factor FaRIF controls fruit ripening in strawberry. Plant Cell. 2021 Jul 2;33(5):1574-1593. doi: 10.1093/plcell/koab070. PMID: 33624824; PMCID: PMC8254488.
NO This work was supported by grants from the European Research Council (ERC-2014-StG 638134 to D.P.) and theSpanish Ministries of Economy and Competitiveness (MINECO, BIO2013-44199-R to V.V.) and of Science andInnovation (MICINN, RTI2018-09309-A-I00 to D.P., BIO2017- 82609-R to M.A.B, and PID2019-111496RR-I00 to I.A.). D.P. and S.O. were supported by the Ramón y Cajal Programs RYC2013-12699 and RYC2011-09170, respectively (MINECO and MICINN, Spain). We thank Plan Propio from the University of Ma´laga for financial support.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026