RT Journal Article
T1 Genomic analysis of the marine fish pathogen Photobacterium damselae subsp. piscicida: Insertion sequences proliferation is associated with chromosomal reorganisations and rampant gene decay.
A1 Balado, Miguel
A1 Benzekri, Hicham
A1 Labella Vera, Alejandro Manuel
A1 Claros-Díaz, Manuel Gonzalo
A1 Manchado, Manuel
A1 Borrego-García, Juan José
A1 Osorio, Carlos
A1 Lemos, Manuel
K1 Peces - Agentes patógenos
AB Photobacterium damselae subsp. piscicida (Pdp) is an intracellular fish pathogen that causes photobacteriosis, adisease proven deadly in farmed fish worldwide. This work focuses on the analysis of genome sequences, chromosomesstructure and gene contents of two strains fromSparus aurata (DI21) and Solea senegalensis (L091106-03H), isolated on the Spanish Atlantic coast. The comparative genomic analysis revealed that DI21 and L091106-03H share 98% of their genomes, including two virulence plasmids: pPHDP70 encoding siderophore piscibactinsynthesis and pPHDP10 encoding the apoptotic toxin AIP56. Both genomes harbour a surprisingly large numberof IS elements accounting for 12–17% of the total genome, representing an IS density of 0.15 elements per kb, oneof the highest IS density values in a bacterial pathogen. This massive proliferation of ISs is responsible for the generationof a high number of pseudogenes that caused extensive loss of biological functions. Pseudogene formationis one of the main features of Pdp genome that explains most of the ecological and phenotypic differences withrespect to its sibling subspecies P. damselae subsp. damselae and to other Vibrionaceae. Evidence was alsofound proving the existence of two chromosomal configurations depending on the origin of the strains: an Europeanand an Asian/American types of genome organisation, reinforcing the idea of the existence of two geographically-linked clonal lineages in Pdp. In short, our study suggests that the host-dependent lifestyle of Pdp allowedmassive IS proliferation and gene decay processes, which are major evolutionary forces in the shaping of the Pdpgenome.
PB Elsevier
YR 2017
FD 2017
LK https://hdl.handle.net/10630/34535
UL https://hdl.handle.net/10630/34535
LA eng
NO Balado M, Benzekri H, Labella AM, Claros MG, Manchado M, Borrego JJ, Osorio CR, Lemos ML. Genomic analysis of the marine fish pathogen Photobacterium damselae subsp. piscicida: Insertion sequences proliferation is associated with chromosomal reorganisations and rampant gene decay. Infect Genet Evol. 2017 Oct;54:221-229. doi: 10.1016/j.meegid.2017.07.007. Epub 2017 Jul 5. PMID: 28688976.
NO https://v2.sherpa.ac.uk/id/publication/16825
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026