RT Conference Proceedings T1 A Psl-like polysaccharide has a key role in the biofilm architecture of two plant-associated Pseudomonas A1 Heredia Ponce, Zaira María A1 Purtschert, G. A1 Gutiérrez Barranquero, José Antonio A1 Eberl, Leo A1 De-Vicente-Moreno, Antonio A1 Cazorla-López, Francisco Manuel AB The Psl polysaccharide has only been studied in Pseudomonas aeruginosa, and its role in bacteria that interact with plants is still unknown. On the one hand, P. syringae is a model of study of plant-pathogen interactions. On the other hand, P. chlororaphis is a biocontrol agent of plant-fungal diseases, as the white root rot caused by Rosellinia necatrix. In silico analysis have let us identify in both species a genomic region which is ortholog to the Psl-encoding region of P. aeruginosa PAO1. We constructed mutants of this region in both strains and analysed its phenotype in biofilm formation, both in static microwell plates and dynamic flow-cell chamber experiments. The results revealed a different and an important role of this polysaccharide in the biofilm architecture of both strains. Furthermore, in P. syringae this Psl-like polysaccharide has a very important role in swarming motility. All these striking phenotypes led us further study the implication of this polysaccharide in the lifestyles of both models of plant-bacteria interaction. PB IS-MPMI YR 2019 FD 2019-07 LK https://hdl.handle.net/10630/18174 UL https://hdl.handle.net/10630/18174 LA eng NO Heredia-Ponce et a., 2019. A Psl-like polysaccharide has a key role in the biofilm architecture of two plant-associated Pseudomonas. ISMPMI 2019 Proceeding book, 854-22 NO Comunicación congreso NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.AGL2017-83368-C2-1-R DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 20 ene 2026