RT Journal Article
T1 Comparative analysis of single-cell transcriptomics in human and zebrafish oocytes
A1 Can, Handan
A1 Chanumolu, Sree K.
A1 González-Muñoz, María Elena
A1 Prukudom, Sukumal
A1 Otu, Hasan H.
A1 Cibelli, Jose B
K1 Ovulos - Aspectos genéticos
K1 Ingeniería genética
AB Background: Zebrafish is a popular model organism, which is widely used in developmental biology research.Despite its general use, the direct comparison of the zebrafish and human oocyte transcriptomes has not been wellstudied. It is significant to see if the similarity observed between the two organisms at the gene sequence level isalso observed at the expression level in key cell types such as the oocyte.Results: We performed single-cell RNA-seq of the zebrafish oocyte and compared it with two studies that haveperformed single-cell RNA-seq of the human oocyte. We carried out a comparative analysis of genes expressed in theoocyte and genes highly expressed in the oocyte across the three studies. Overall, we found high consistency betweenthe human studies and high concordance in expression for the orthologous genes in the two organisms. According tothe Ensembl database, about 60% of the human protein coding genes are orthologous to the zebrafish genes. Ourresults showed that a higher percentage of the genes that are highly expressed in both organisms show orthologycompared to the lower expressed genes. Systems biology analysis of the genes highly expressed in the three studiesshowed significant overlap of the enriched pathways and GO terms. Moreover, orthologous genes that are commonlyoverexpressed in both organisms were involved in biological mechanisms that are functionally essential to the oocyte.Conclusions: Orthologous genes are concurrently highly expressed in the oocytes of the two organisms and thesegenes belong to similar functional categories. Our results provide evidence that zebrafish could serve as a valid modelorganism to study the oocyte with direct implications in human.
PB Springer Nature
YR 2020
FD 2020-07-08
LK https://hdl.handle.net/10630/38829
UL https://hdl.handle.net/10630/38829
LA eng
NO Can, H., Chanumolu, S.K., Gonzalez-Muñoz, E. et al. Comparative analysis of single-cell transcriptomics in human and zebrafish oocytes. BMC Genomics 21, 471 (2020). https://doi.org/10.1186/s12864-020-06860-z
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026