RT Journal Article
T1 Magnetically active pNIPAM nanosystems as temperature-sensitive biocompatible structures for controlled drug delivery
A1 García-Pinel, Beatriz
A1 Ortega Rodríguez, Alicia
A1 Porras Alcalá, Cristina
A1 Cabeza, Laura
A1 Contreras-Cáceres, Rafael
A1 Ortiz, Raúl
A1 Díaz-Morilla, Amelia
A1 Moscoso, Ana
A1 Sarabia-García, Francisco Ramón
A1 Prados, Jose
A1 López-Romero, Juan Manuel
A1 Melguizo, Consolación
K1 Química farmacéutica
AB Here, temperature-sensitive hybrid poly(N-isopropylacrylamide) (pNIPAM) nanosystems with magnetic response are synthesised and investigated for controlled release of 5-fluorouracil (5FU) and oxaliplatin (OXA). Initially, magnetic nanoparticles (@Fe3O4) are synthesised by co-precipitation approach and functionalised with acrylic acid (AA), 3-butenoic acid (3BA) or allylamine (AL) as comonomers. The thermo-responsive polymer is grown by free radical polymerisation using N-isopropylacrylamide (NIPAM) as monomer, N,N’-methylenbisacrylamide (BIS) as cross-linker, and 2,2’-azobis(2-methylpropionamidene) (V50) as initiator. We evaluate particle morphology by transmission electron microscopy (TEM) and particle size and surface charge by dynamic light scattering (DLS) and Z-potential (ZP) measurements. These magnetically active pNIPAM@ nanoformulations are loaded with 5-fluorouracil (5FU) and oxaliplatin (OXA) to determine loading efficiency, drug content and release as well as the cytotoxicity against T-84 colon cancer cells. Our results show high biocompatibility of pNIPAM nanoformulations using human blood cells and cultured cells. Interestingly, the pNIPAM@Fe3O4-3BA + 5FU nanoformulation significantly reduces the growth of T-84 cells (57% relative inhibition of proliferation). Indeed, pNIPAM-co-AL@Fe3O4-AA nanosystems produce a slight migration of HCT15 cells in suspension in the presence of an external magnetic field. Therefore, the obtained hybrid nanoparticles can be applied as a promising biocompatible nanoplatform for the delivery of 5FU and OXA in the improvement of colon cancer treatments.
PB Taylor and Francis
YR 2020
FD 2020-04-19
LK https://hdl.handle.net/10630/29408
UL https://hdl.handle.net/10630/29408
LA eng
NO Beatriz Garcia-Pinel, Alicia Ortega-Rodríguez, Cristina Porras-Alcalá, Laura Cabeza, Rafael Contreras-Cáceres, Raul Ortiz, Amelia Díaz, Ana Moscoso, Francisco Sarabia, José Prados, Juan M. López-Romero & Consolación Melguizo (2020) Magnetically active pNIPAM nanosystems as temperature-sensitive biocompatible structures for controlled drug delivery, Artificial Cells, Nanomedicine, and Biotechnology, 48:1, 1022-1035, DOI: 10.1080/21691401.2020.1773488
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026