RT Conference Proceedings
T1 Isolation, characterization and selection of Pseudomonas spp. as biological control agents from a suppressive soil
A1 Vida Hinojosa, Carmen María
A1 Tienda Serrano, Sandra
A1 Cazorla-López, Francisco Manuel
A1 De-Vicente-Moreno, Antonio
K1 Pseudomonas
K1 Aguacates
AB Bacterial profile from a suppressive soil against Rosellinia necatrix, fungal pathogen of avocado roots, were obtained by 16S rRNA gene sequencing. The results revealed a significant increase in the bacterial class of Gammaproteobacteria, especially in some antagonistic representatives of Pseudomonas spp. For this reason, a collection of 246 bacterial isolates was obtained from this suppressive soil in order to identify new strains with antifungal activity against this fungal phytopathogen. First, we performed an isolation on a selective medium for Pseudomonas-like microorganisms. Then, we used different characterization tests in order to analyse the bacterial collection, including the identification of the general metabolic profile of glucose, the profiling of antifungals produced, both the putative production of antifungal compounds and lytic exoenzymes, as well as the evaluation of traits related with beneficial effects on plants. A final selection of representative strains resulted in antifungal isolates belonging to the genus Pseudomonas and related groups. These selected strains were tested for plant protection by an in vivo experiment using avocado and wheat plants challenged by the pathogen R. necatrix, showing all of them an antifungal ability and plant disease protection.
YR 2017
FD 2017-09-21
LK http://hdl.handle.net/10630/14499
UL http://hdl.handle.net/10630/14499
LA eng
NO This work was supported by Plan Nacional I+D+I from MINECO (Spain) (AGL2014-52518-C2-IR) and co-financed by FEDER funds (EU). C.Vida was supported by a PhD fellowship from the FPI program of MINECO.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026