RT Conference Proceedings
T1 Genetically engineering Pseudomonas strains for T6SS-dependent controlled release of effectors.
A1 Araujo-Garrido, Mario
A1 Pérez-Lorente, Alicia Isabel
A1 Romero-Hinojosa, Diego Francisco
A1 Molina-Santiago, Carlos
K1 Bacterias fitopatógenas
AB Type VI secretion systems (T6SS) are Gram-negative bacteria’s syringe-like nanomachine that injects effectors into prokaryotic and eukaryotic cells leading to thedeath of their target. These systems have the potential to be genetically engineered for their application in different biotechnological fields such as medicine oragriculture. As proof of concept, in this work we have genetically engineered the well-known soil-dwelling Pseudomonas putida KT2440 strain, achieving thecontrolled expression of related and non-related T6SS effectors. These results open a window of opportunities to secrete a wide range of molecules to differenttargets using this Pseudomonas strain as bacterial chassis. Specifically, this work shows promising results in the agriculture field as genetically modified P. putidaKT2440 strains overexpressing T6SS-related effector Tfe2 and T6SS-non-related effector chitosanase can now inhibit bacterial and fungal strains such as thephytopathogen Botrytis cinerea.
YR 2024
FD 2024
LK https://hdl.handle.net/10630/31949
UL https://hdl.handle.net/10630/31949
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026