RT Conference Proceedings
T1 Analysis of gene expression in nodavirus-inoculated Senegalese sole using a new Openarray® platform.
A1 Labella Vera, Alejandro Manuel
A1 Gémez Mata, Juan
A1 Vera, Isabel
A1 Bandin, Isabel
A1 Leiva Rebollo, Rocío
A1 Borrego-García, Juan José
A1 García-Rosado, Esther
K1 Peces - Agentes patógenos
AB Nervous necrosis virus (NNV) is the causative agent of the viral encephalopathyand retinopathy, a disease that affects cultured Senegalese sole (Solea senegalensis). A NNV reassortant (Ss160.03), combining genomicsegments from red-spotted grouper nervous necrosis virus (RGNNV) andstriped jack nervous necrosis virus (SJNNV) genotypes, has been previouslyisolated from Senegalese sole, being highly virulent to this fish species. TheRNA-Seq technology has been used in a previous study to comparativelyanalyse Senegalese sole transcriptomes in two organs (head kidney andeye/brain) after infection with two NNV virus with different levels ofvirulence to that fish species, a highly virulent reassortant isolate(wSs160.03) and a less virulent mutant reassortant obtained by reversegenetics (rSs160.03247þ270). To validate previous RNA-Seq results, a 112-assay OpenArray® platform (Thermofisher) has been designed. This platformincluded 89 genes chosen according to transcriptomic changesobserved by RNA-Seq (covering PRRs, type I IFN response, signal transduction,inflammation, virus responsive genes, and apoptosis), 17 genesselected based on their previously described relation with the immuneresponse against fish viral infections, and 6 control genes (including 3endogenous genes and 3 viral genes).
PB Elsevier
YR 2019
FD 2019
LK https://hdl.handle.net/10630/34600
UL https://hdl.handle.net/10630/34600
LA eng
NO A. Labella, J. Gémez, I. Vera, I. Bandín, R. Leiva, J.J. Borrego, E. Garcia-Rosado, Analysis of gene expression in nodavirus-inoculated Senegalese sole using a new Openarray® platform, Fish & Shellfish Immunology, Volume 91, 2019, Pages 391-392, ISSN 1050-4648, https://doi.org/10.1016/j.fsi.2019.04.077. (https://www.sciencedirect.com/science/article/pii/S1050464819303158)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026