RT Journal Article
T1 Pseudomonas savastanoi pv. mandevillae pv. nov., a Clonal Pathogen Causing an Emerging, Devastating Disease of the Ornamental Plant Mandevilla spp.
A1 Caballo-Ponce, Eloy
A1 Pintado Calvillo, Adrián
A1 Moreno-Pérez, Alba
A1 Murillo, Jesús
A1 Smalla, Kornelia
A1 Ramos-Rodríguez, Cayo Juan
K1 Pseudomonas syringae - Genética
K1 Bacterias fitopatógenas - Aspectos genéticos
K1 Bioinformática
AB Commercial production of the ornamental plant dipladenia (Mandevilla spp.) is threatened by dipladenia leaf and stem spot disease, caused by the bacterium Pseudomonas savastanoi. P. savastanoi includes four pathovars of woody hosts differentiated by a characteristic host range in olive, oleander, ash, and broom plants. However, isolates from dipladenia have not been ascribed to any particular lineage or P. savastanoi pathovar. Here we report that isolates from dipladenia represent a distinct, clonal lineage. First, dipladenia isolates display very similar plasmid profiles, including a plasmid encoding the iaaM gene for biosynthesis of indole-3-acetic acid. Second, multilocus sequence analysis and core genome single-nucleotide polymorphisms phylogenies showed a monophyletic origin for dipladenia isolates, which cluster with isolates from oleander (pathovar nerii) in a distinct clade well separated from other P. savastanoi strains. Metabolic profiling and cross-pathogenicity tests in olive, oleander, ash, broom, and dipladenia clearly distinguished dipladenia isolates from the four P. savastanoi pathovars. Comparative genomics of the draft genome sequence of the dipladenia strain Ph3 with the other four pathovars showed that Ph3 encodes very few strain-specific genes and a similar set of virulence genes to pv. nerii, including its repertoire of type III secretion system effectors. However, hierarchical clustering based on the catalog of effectors and their allelic variants clearly separated Ph3 from pv. nerii strains. Based on their distinctive pathogenicity profile, we propose a de novo pathovar for P. savastanoi isolates from dipladenia, P. savastanoi pv. mandevillae pv. nov., for which strain Ph3 (CFBP 8832PT) has been designated as the pathotype strain.
PB The American Phytopathological Society (APS)
YR 2021
FD 2021-09-11
LK https://hdl.handle.net/10630/39010
UL https://hdl.handle.net/10630/39010
LA eng
NO Phytopathology (2021), 111: 1277-1288
NO Esta es la versión revisada por pares del siguiente artículo: Caballo-Ponce et al., 2021, Phytopathology, 111:1277-1288, que ha sido publicado en su forma definitiva en https://doi.org/10.1094/PHYTO-11-20-0526-R. Este artículo puede usarse para fines no comerciales conforme a los Términos y Condiciones de Wiley para el uso de versiones autoarchivadas. A este documento se aplicó un embargo de 12 meses (concluido en 2021-06-13) según la política de autoarchivo de Wiley para versiones aceptadas de artículos publicados en Phytopathology. Esta restricción protege los derechos comerciales y de distribución de la editorial, permitiendo la publicación abierta del manuscrito tras dicho período.Fuente: Política de autoarchivo de Wiley, disponible en https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html
NO https://openpolicyfinder.jisc.ac.uk/id/publication/10350
NO Ministerio de Ciencia, Innovación y Universidades (Spain)Grant/Award Number: FPI/BES-2012-052398Grant/Award Number: FPI/BES-2015-074847Grant/Award Number: FPU14/05551Grant/Award Number: AGL2017-82492-C2-1-RGrant/Award Number: AGL2017-82492-C2-2-REuropean Regional Development Fund
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026