RT Journal Article
T1 Structural and functional characteristics of two molecular variants of the nitrogen sensor PII in maritime pine.
A1 Llebrés, María Teresa
A1 Pascual-Moreno, María Belén
A1 Valle, Carolina
A1 De-la-Torre-Fazio, Fernando Nicolás
A1 Valderrama-Martín, José Miguel
A1 Gómez, Luis
A1 Ávila-Sáez, Concepción
A1 Cánovas-Ramos, Francisco Miguel
K1 Plantas - Efectos del nitrógeno
K1 Pinos - Metabolismo
AB High levels of nitrogen are stored as arginine during the last stages of seedformation in maritime pine (Pinus pinaster Aiton). The protein sensor PII regulatesthe feedback inhibition of arginine biosynthesis through interaction with the keyenzyme N-acetylglutamate kinase (NAGK). In this study, the structural and functionalcharacteristics of PII have been investigated in maritime pine to get insights into theregulation of arginine metabolism. Two different forms of PII have been identified, PpPIIaand PpPIIb, which differ in their amino acid sequence and most likely correspond tosplicing variants of a single gene in the pine genome. Two PII variants are also presentin other pine species but not in other conifers such as spruces. PpPIIa and PpPIIb aretrimeric proteins for which structural modeling predicts similar tridimensional protein corestructures. Both are located in the chloroplast, where the PII-target enzyme PpNAGKis also found. PpPIIa, PpPIIb, and PpNAGK have been recombinantly produced toinvestigate the formation of NAGK-PII complexes. The interaction of PpPIIa/PpPIIband PpNAGK may be enhanced by glutamine and contribute to relieve the feedbackinhibition of PpNAGK by arginine. Expression analysis of PpPII genes revealed that PpIIatranscripts were predominant during embryogenesis and germination. The potentialroles of PpPIIa and PpPIIb in the regulation of arginine metabolism of maritime pineare discussed.
PB Frontiers
SN 1664-462X
YR 2020
FD 2020
LK https://hdl.handle.net/10630/37920
UL https://hdl.handle.net/10630/37920
LA eng
NO Llebrés MT, Pascual MB, Valle C, de la Torre FN, Valderrama-Martin JM, Gómez L, Avila C and Cánovas FM (2020) Structural and Functional Characteristics of Two Molecular Variants of the Nitrogen Sensor PII in Maritime Pine. Front. Plant Sci. 11:823. doi: 10.3389/fpls.2020.00823
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026