RT Journal Article
T1 GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi
A1 Lavado-Benito, Carla
A1 Murillo, Jesús
A1 Martínez Gil, Marta
A1 Ramos-Rodríguez, Cayo Juan
A1 Rodríguez-Moreno, Luis Gabriel
K1 Pseudomonas syringae - Genética
K1 Bacterias fitopatógenas
K1 Bacterias gram negativas
AB GacS/GacA is a widely distributed two-component system playing an essentialrole as a key global regulator, although its characterization in phytopathogenicbacteria has been deeply biased, being intensively studied in pathogens ofherbaceous plants but barely investigated in pathogens of woody hosts. P.savastanoi pv. savastanoi (Psv) is characterized by inducing tumours in thestem and branches of olive trees. In this work, the model strain Psv NCPPB3335 and a mutant derivative with a complete deletion of gene gacA weresubjected to RNA-Seq analyses in a minimum medium and a medium mimickingin planta conditions, accompanied by RT-qPCR analyses of selected genes andphenotypic assays. These experiments indicated that GacA participates in theregulation of at least 2152 genes in strain NCPPB 3335, representing 37.9 % of theannotated CDSs. GacA also controls the expression of diverse rsm genes, andmodulates diverse phenotypes, including motility and resistance to oxidativestresses. As occurs with other P. syringae pathovars of herbaceous plants, GacAregulates the expression of the type III secretion system and cognate effectors. Inaddition, GacA also regulates the expression of WHOP genes, specificallyencoded in P. syringe strains isolated from woody hosts, and genes for thebiosynthesis of phytohormones. A gacA mutant of NCPPB 3335 showedincreased virulence, producing large immature tumours with high bacterialpopulations, but showed a significantly reduced competitiveness in planta. Ourresults further extend the role of the global regulator GacA in the virulence andfitness of a P. syringae pathogen of woody hosts
PB Frontiers Media
YR 2024
FD 2024-02-05
LK https://hdl.handle.net/10630/39007
UL https://hdl.handle.net/10630/39007
LA eng
NO Front. Plant Sci. 15:1347982
NO The author(s) declare financial support was received for theresearch, authorship, and/or publication of this article. Thisresearch was supported by project grants PID2020-115177RB-C21and PID2020-115177RB-C22 financed by the Spanish Ministry ofScience and Innovation (MCIN)/Agencia Estatal de Investigacioń(AEI)/10.13039/501100011033/ and by the European Regional Development Fund (ERDF) “A way to make Europe”. CL-B wassupported by the FPI2018-084276 predoctoral grant. Open accesswas partially funded by grant QUAL21 012 IHSM, Consejería deUniversidad, Investigación e Innovación, Junta de Andalucıa.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026