RT Journal Article
T1 Contribution of mangotoxin to the virulence and epiphytic fitness of Pseudomonas syringae pv. syringae.
A1 Arrebola-Díez, Eva María
A1 Cazorla-López, Francisco Manuel
A1 Codina, Juan C.
A1 Gutiérrez Barranquero, José Antonio
A1 Pérez-García, Alejandro
A1 De-Vicente-Moreno, Antonio
K1 Fitotoxinas
K1 Pseudomonas syringae
K1 Antimetabolitos
K1 Plantas - Enfermedades y plagas - Patogénesis
AB Mangotoxin is an antimetabolite toxin that inhibits ornithine acetyl transferase, a key enzyme in the biosyntheticpathway of ornithine and arginine and recently reported in strains of Pseudomonas syringae pv. syringae (Pss) isolated frommango. Since symptoms on mango tissues are very difficult to reproduce, in this study the role of mangotoxin in Pss virulencewas addressed by analyzing the in planta growth and development of disease symptoms on tomato leaflets. Inoculationexperiments were carried out following several procedures using the wild-type strain Pss UMAF0158, two Tn5-mutant derivativestrains defective in mangotoxin production, and their complemented derivative strains in which mangotoxin productionis restored. The ability of the mangotoxin-defective mutants to grow in planta was similar, and their epiphytic survival on thetomato leaf surface identical to the wild-type and complemented strains. However, both the disease index data of incidenceand the severity of necrotic symptoms indicated that mangotoxin-defective mutants were less virulent, indicating that mangotoxinis a virulence factor. Furthermore, competition experiments showed that the survival values of the wild-type strainwere slightly but significantly higher than those of the mangotoxin-defective mutants, suggesting that mangotoxin productionwould improve the epiphytic fitness of Pss.
PB Institut d’Estudis Catalans
YR 2009
FD 2009-06-10
LK https://hdl.handle.net/10630/30982
UL https://hdl.handle.net/10630/30982
LA eng
NO Arrebola E, Cazorla FM, Codina JC, Gutiérrez-Barranquero JA, Pérez-García A, de Vicente A. Contribution of mangotoxin to the virulence and epiphytic fitness of Pseudomonas syringae pv. syringae. Int Microbiol. 2009. 12(2):87-95.
NO https://revistes.iec.cat/index.php/IM/about/submissions
NO This work was partially supported by grantsfrom the CICE-Andalusian Autonomous Government (Group AGR-169 andProject P07-AGR-2471) and from R+D National Plan from the SpanishMinistry of Science and Innovation (AGL2005-06347-C03-01).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026