RT Conference Proceedings
T1 The dopaminergic mesolimbic system are involved in the anhedonic-like effects produced by galanin (1-15) in rats.
A1 Cantero-García, Noelia
A1 García-Durán, Laura
A1 Gago-Calderón, Belén
A1 Flores-Burgess, Antonio
A1 Alén, Francisco
A1 Orio, Laura
A1 Narváez-Bueno, José Ángel
A1 Fuxe, Kjell
A1 Santín-Núñez, Luis Javier
A1 Díaz-Cabiale, Zaida
A1 Millón-Peñuela, Carmelo
K1 Depresión mental
AB Galanin (1-15)[GAL(1-15)] induces depressant- and anxiogenic-like actions in several behavioural tests. Recently, we described that GAL(1-15) induces an anhedonic-like effect in saccharine self-administration and sucrose preference test in rats. In order to investigate whether the effect of GAL(1–15) in anhedonia was associated with the reward circuit, we have studied the GAL(1-15) actions over the mesolimbic system by PET for in vivo imaging and in the expression of the C-Fos, Dat, Vmat2 and Dopamine and Galanin receptors genes in VTA and NAc.In the PET experiments, the [18F]FDG at 30, 60 and 90min after GAL(1-15) administration was measure as indicative of brain glucose metabolism. In the qPCR experiments, groups of rats (n=5-6) were killed 1h after i.c.v. GAL(1-15) 3nmol or vehicle. The VTA and NAc were dissected and the mRNA expression of C-Fos, Dat, Vmat2 and D1, D2, D3, D5, GALR1, and GALR2 receptors were measured by RT-qPCR.The GAL(1-15) induced a decrease in [18F]FDG uptake in the hippocampus and thalamus at 30, 60 and 90min, and in the striatum at 30min, however, in the prefrontal cortex, an increase in [18F]FDG uptake was observed after 30 and 60min. GAL(1-15) at a dose of 3 nmol produced a significant decrease in the mRNA levels of Dat and Vmat2 (p<0.05) and an increase in the D3 receptor (p<0.05) in VTA. In the NAc, GAL(1-15) induced a significant decrease in the expression of C-Fos (p<0.05) mRNA and a significantly increased the mRNA expression of D1 (p<0.05), D2 (p<0.05) and D3 (p<0.05).These results suggest the involvement of the dopaminergic mesolimbic system, a key region for the reward system, in GAL(1-15)-mediated action on anhedonia. These results may give the basis for the development of novel therapeutic strategies using GAL(1-15) for treatment of depression and reward-related diseases.
YR 2019
FD 2019-09-10
LK https://hdl.handle.net/10630/18290
UL https://hdl.handle.net/10630/18290
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.This study was supported by SpanishSAF2016-79008 and PPIT.UMA.B1.2017/17.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026