RT Journal Article
T1 Deregulation of phenylalnine biosynthesis evolved with the emergence of vascular plants.
A1 El-Azaz-Ciudad, Jorge
A1 Cánovas-Ramos, Francisco Miguel
A1 Barcelona, Belén
A1 Ávila-Sáez, Concepción
A1 De-la-Torre-Fazio, Fernando Nicolás
K1 Fenilalanina
K1 Plantas - Sistema vascular
AB Phenylalanine (Phe) is the precursor of essential secondary products in plants. Here we show that a key, rate-limiting step in Phe biosynthesis, which is catalyzed by arogenate dehydratase, experienced feedback de-regulation during evolution. Enzymes from microorganisms and type-I ADTs from plants are strongly feedback-inhibited by Phe, while type-II isoforms remain active at high levels of Phe. We have found that type-II ADTs are widespread across seed plants and their overproduction resulted in a dramatic accumulation of Phe in planta, reaching levels up to 40 times higher than those observed following the expression of type-I enzymes. Punctual changes in the allosteric binding site of Phe and adjacent region are responsible for the observed relaxed regulation. The phylogeny of plant ADTs evidences that the emergence of type-II isoforms with relaxed regulation occurred at some point in the transition between nonvascular plants and tracheophytes, enabling the massive production of Phe-derived compounds, primarily lignin, a hallmark of vascular plants.
PB Oxford University Press
SN 0140-7791
YR 2022
FD 2022
LK https://hdl.handle.net/10630/37926
UL https://hdl.handle.net/10630/37926
LA eng
NO Jorge El-Azaz, Francisco M Cánovas, Belén Barcelona, Concepción Ávila, Fernando de la Torre, Deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plants, Plant Physiology, Volume 188, Issue 1, January 2022, Pages 134–150, https://doi.org/10.1093/plphys/kiab454
NO This work was supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICINN) (BIO2015-69285-R and RTI2018-094041-B-I00) and Junta Andalucía (Research Group BIO-114).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026