RT Journal Article
T1 Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
A1 Carmona Martín, Elisabeth
A1 Regalado, José Javier
A1 Perán-Quesada, Rosa Amelia
A1 López Encina, Carlos
K1 Espárragos
K1 Crioconservación
K1 Marcadores biológicos
AB We describe an encapsulation–dehydration procedure with prefreezing steps for the cryopreservation of rhizome bud explants of Asparagus officinalis L. cv. Morado de Huétor. With this procedure, survival of Rhizome buds was at least 84 and 42% developed to complete plantlets at 8 weeks. Flow cytometry and EST-SSR molecular markers were used to assess genetic stability of the regenerated material. Effects of preculture time in a medium rich in sucrose and prefreezing treatments (0 °C or/and − 20 °C) on plant recovery were evaluated. Rhizome Buds of the “Morado de Huétor” landrace were incubated in preculture medium (MS + 0.3 M sucrose) for 48 h, encapsulated in alginate beads and desiccated until a water content of 35%, prefrozen for one hour at 0 °C plus one hour at − 20 °C, followed by cryopreservation in liquid nitrogen, and then were rewarmed and recovered in ARBM medium for 6 weeks and finally incubated in ARBM-0 for 4 weeks. Analyses of ploidy and molecular stability of plantlets recovered from cryopreserved rhizome buds of two selected genotypes showed no differences compared with the mother plants. Cryopreservation of RB explants of A. officinalis with this Encapsulation–Dehydration procedure will be useful in long-term preservation programs.
PB Springer Nature
SN 1573-5044
YR 2018
FD 2018-02-09
LK https://hdl.handle.net/10630/45141
UL https://hdl.handle.net/10630/45141
LA eng
NO Carmona-Martín, E., Regalado, J.J., Perán-Quesada, R. et al. Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability. Plant Cell Tiss Organ Cult 133, 395–403 (2018). https://doi.org/10.1007/s11240-018-1392-y
NO https://openpolicyfinder.jisc.ac.uk/id/publication/16214?from=single_hit
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 1 mar 2026