RT Journal Article
T1 Functional Analysis of Gene-Silencing Suppressors from Tomato Yellow Leaf Curl Disease Viruses
A1 Pérez-Luna, Ana Isabel
A1 Morilla, Gabriel
A1 Voinnet, Olivier
A1 Rodríguez-Bejarano, Eduardo
K1 Virus fitopatógenos
AB Tomato yellow leaf curl disease (TYLCD) is caused by acomplex of phylogenetically related Begomovirus spp. thatproduce similar symptoms when they infect tomato plantsbut have different host ranges. In this work, we have evaluatedthe gene-silencing-suppression activity of C2, C4, andV2 viral proteins isolated from the four main TYLCDcausingstrains in Spain in Nicotiana benthamiana. We observedvarying degrees of local silencing suppression foreach viral protein tested, with V2 proteins from all four virusesexhibiting the strongest suppression activity. None ofthe suppressors were able to avoid the spread of the systemicsilencing, although most produced a delay. In orderto test the silencing-suppression activity of Tomato yellowleaf curl virus (TYLCV) and Tomato yellow leaf curl Sardiniavirus (TYLCSV) proteins in a shared (tomato) andnonshared (bean) host, we established novel patch assays.Using these tools, we found that viral proteins from TYLCVwere able to suppress silencing in both hosts, whereasTYLCSV proteins were only effective in tomato. This is thefirst time that viral suppressors from a complex of diseasecausinggeminiviruses have been subject to a comprehensiveanalysis using two economically important crop hosts,as well as the established N. benthamiana plant model.
PB APS Publications
YR 2012
FD 2012-06-01
LK https://hdl.handle.net/10630/34173
UL https://hdl.handle.net/10630/34173
LA eng
NO Functional Analysis of Gene-Silencing Suppressors from Tomato Yellow Leaf Curl Disease Viruses Ana P. Luna, Gabriel Morilla, Olivier Voinnet, and Eduardo R. Bejarano Molecular Plant-Microbe Interactions® 2012 25:10, 1294-1306
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026