RT Journal Article
T1 A Nonribosomal Peptide Synthetase Gene (mgoA) of Pseudomonas syringae pv. syringae Is Involved in Mangotoxin Biosynthesis and Is Required for Full Virulence.
A1 Arrebola-Díez, Eva María
A1 Cazorla-López, Francisco Manuel
A1 Romero-Hinojosa, Diego Francisco
A1 Pérez-García, Alejandro
A1 De-Vicente-Moreno, Antonio
K1 Fitotoxinas
K1 Mangos - Enfermedades y plagas
K1 Antimetabolitos
K1 Bacterias fitopatógenas
AB Pseudomonas syringae pv. syringae, which causes the bacterialapical necrosis of mango, produces the antimetabolitemangotoxin. We report here the cloning, sequencing, andidentity analysis of a chromosomal region of 11.1 kb fromstrain P. syringae pv. syringae UMAF0158, which is involvedin mangotoxin biosynthesis. This chromosomal region containssix complete open reading frames (ORFs), including alarge gene (ORF5) with a modular architecture characteristicof nonribosomal peptide synthetases (NRPS) namedmgoA. A Tn5 mutant disrupted in mgoA was defective inmangotoxin production, revealing the involvement of theputative NRPS gene in the biosynthesis of mangotoxin.This derivative strain impaired in mangotoxin productionalso showed a reduction in virulence as measured by necroticsymptoms on tomato leaflets. Mangotoxin productionand virulence were restored fully in the NRPS mutantby complementation with plasmid pCG2-6, which containsan 11,103-bp chromosomal region cloned from the wildtypestrain P. syringae pv. syringae UMAF0158 that includesthe putative NPRS gene (mgoA). The results demonstratethat mgoA has a role in the virulence of P. syringae pv.syringae. The involvement of an NRPS in the production ofan antimetabolite toxin from P. syringae inhibiting ornithineacetyltransferase activity is proposed.
PB The American Phytopathological Society (APS)
YR 2007
FD 2007-04-20
LK https://hdl.handle.net/10630/30984
UL https://hdl.handle.net/10630/30984
LA eng
NO Arrebola E, Cazorla FM, Romero D, Pérez-García A, de Vicente A. A nonribosomal peptide synthetase gene (mgoA) of Pseudomonas syringae pv. syringae is involved in mangotoxin biosynthesis and is required for full virulence. Mol Plant Microbe Interact. 2007. 20(5):500-9.
NO Política de acceso abierto tomada de: https://v2.sherpa.ac.uk/id/publication/10349 -- https://apsjournals.apsnet.org/page/open_access
NO This work was partially supported by grants from the Junta deAndalucía (AGR-169) and from Plan Nacional de I+D del Ministerio deEducación y Ciencia, PETRI PTR1995-0723-OP, AGL2004-06056-C02-01,and AGL2004-07028-CO3-02.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026