RT Journal Article
T1 Disentangling the molecular mechanisms of disease suppression by endophytic Flavobacterium sp. 98
A1 Pan, Xinya
A1 Elsayed, Somayah S.
A1 van Wezel, Gilles P.
A1 Raaijmakers, Jos M.
A1 Carrión, Víctor J.
K1 Flavobacterium
K1 Suelos supresivos
K1 Microbioma endofítico
K1 Benzimidazoles
K1 Genómica
AB Endophytic microorganisms colonize internal plant tissues and enhance host resistance to pathogens. We previously showed that endophytic Flavobacterium sp. 98 (Fl98) protects sugar beet against the fungal root pathogen Rhizoctonia solani via biosynthetic gene cluster 298 (BGC298). However, the molecular mechanisms underlying this protection remained poorly understood. Here, comparative metabolomic analyses revealed that knockout of BGC298 led to reduced production of the antifungal compound 5,6-dimethylbenzimidazole (DMB) in Fl98. Wehypothesized that BGC298 is involved in regulating DMB biosynthesis and therefore contributes to Fl98’s diseasesuppression as a novel protective mechanism. Subsequent site-directed mutagenesis of the DMB-synthase gene bluB abolished DMB production by Fl98, and both ΔBGC298 and ΔbluB mutants were compromised in protecting sugar beet seedlings in greenhouse bioassays. Bioinformatic analyses further indicated that bluB is widespread across Flavobacterium, while BGC298 is limited to a small subset of plant-associated strains. Together, our findings highlight the pivotal role of BGC298 and DMB biosynthesis in plant protection by endophytic Flavobacterium sp. 98
PB Elsevier
YR 2025
FD 2025
LK https://hdl.handle.net/10630/41110
UL https://hdl.handle.net/10630/41110
LA eng
NO Pan,S., Elsayed, S., van Wezel, G.P., Raaijmakers,J. M., Carrión,V.J.(2026). Disentangling the molecular mechanisms of disease suppression by endophytic Flavobacterium sp. 98. Microbiological Research, 304 (2026),128415.10.1016/j.micres.2025.128415.
NO Funding for open access charge: Universidad de Málaga/CBUA
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026