RT Journal Article
T1 Dendrimer-Modified Solid Supports: Nanostructured Materials with Potential Drug Allergy Diagnostic Applications
A1 Ruiz-Sanchez, Antonio Jesus
A1 Montañez-Vega, María Isabel
A1 Nájera-Albendín, Francisco
A1 Vida-Pol, Yolanda
A1 Pérez-de-Inestrosa-Villatoro, Ezequiel
K1 Biosensores
AB Complex functional materials consisting of bioactive molecules immobilized on solid supports present potential applications inbiosensoring. Advances in the fabrication of these surface materials are of growing interest for antibody-based diagnosis. This work exploitsdendrimers as versatile nanostructures for templating sensor surfaces and the critical role of the immobilization protocol in thesolid supports cellulose and zeolites, of organic and inorganic composition respectively. The fabrication and characterization, includingthe degree of functionalization and reproducibility, of different nanostructured materials are described. To validate the approach, the fabricatedsupports were further used as a solid phase for developing a radioimmunoassay to detect immunoglobulin E (IgE) specific topenicillin, the antibody involved in immediate allergy responses to this drug. The dendrimer-modified supports provide assays with significantlyenhanced sensitivity, as well as increase the availability of biomolecules for specific interaction and minimize nonspecific adsorptionsthrough appropriate functionalization protocols in each case. The manufacturing methodology involved the use of a long, flexiblehydrophilic spacer in the cellulose materials, and a higher surface density of the immobilized dendrimers in the zeolite crystals. Theability of hybrid zeolite materials in such biosensing applications was evaluated for the first time. The assays were validated in human serumsamples from patients allergic to penicillin and from non-allergic controls. The specificity and improved sensitivity of the dendrimer-modified supports make these strategies versatile for different bioactive molecules and could have significant implications for thequantification of a wide range of specific IgE antibodies and other biomolecules of diagnostic interest.
PB Bentham Science
YR 2012
FD 2012
LK https://hdl.handle.net/10630/33107
UL https://hdl.handle.net/10630/33107
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026