RT Journal Article
T1 Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota.
A1 Garzarella, Emanuele Ugo
A1 Navajas Porras, Beatriz
A1 Pérez-Burillo, Sergio
A1 Ullah, Hammad
A1 Esposito, Cristina
A1 Santarcangelo, Cristina
A1 Hinojosa Nogueira, Daniel
A1 Pastoriza, Silvia
A1 Zaccaria, Vincenzo
A1 Xiao, Jianbo
A1 Rufián-Henares, José Ángel
A1 Daglia, Maria
K1 Acidos grasos
K1 Digestión
K1 Antioxidantes
K1 Flora intestinal
K1 Propóleos
AB Introduction: A large body of evidence suggests that propolis exerts antioxidant, anti-inflammatory, and antimicrobial activities, mostly ascribed to its polyphenol content. Growing evidence suggests that propolis couldmodulate gut microbiota exerting a positive impact on several pathological conditions. The aim of this study wasto determine the in vitro impact of a poplar-type propolis extract with a standardized polyphenol content, on thecomposition and functionality of gut microbiota obtained from fecal material of five different donors (healthyadults, and healthy, obese, celiac, and food allergic children).Methods: The standardized polyphenol mixture was submitted to a simulated in vitro digestion-fermentationprocess, designed to mimic natural digestion in the human oral, gastric, and intestinal chambers. The antioxidant profile of propolis before and after the digestion-fermentation process was determined. 16 S rRNA ampliconnext-generation sequencing (NGS) was used to test the effects on the gut microbiota of propolis extract. Theprofile of the short-chain fatty acids (SCFA) produced by the microbiota was also investigated through a chromatographic method coupled with UV detection.Results: In vitro digestion and fermentation induced a decrease in the antioxidant profile of propolis (i.e., decreaseof total polyphenol content, antiradical and reducing activities). Propolis fermentation exhibited a modulatoryeffect on gut microbiota composition and functionality of healthy and diseased subjects increasing the concentration of SCFA.Conclusions: Overall, these data suggest that propolis might contribute to gut health and could be a candidate forfurther studies in view of its use as a prebiotic ingredient.
PB Elsevier
YR 2022
FD 2022-04
LK https://hdl.handle.net/10630/38613
UL https://hdl.handle.net/10630/38613
LA eng
NO Garzarella EU, Navajas-Porras B, Pérez-Burillo S, Ullah H, Esposito C, Santarcangelo C, et al. Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota. Biomedicine & Pharmacotherapy. 2022;148:112759.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026