RT Conference Proceedings
T1 An evolutionary approach to detect the maximum potential adaptation of Microscystis aeruginosa to salinity
A1 Melero-Jiménez, Ignacio José
A1 Martín-Clemente, Elena
A1 García-Sánchez, María Jesús
A1 Flores-Moya, Antonio
A1 Bañares-España, Elena
K1 Cianobacterias
AB Backgrounds Salinity is increasing in many inland waters, on a world-wide scale, due to agricultural practices, droughts, or rise in sea level. In order to understand the effects of increased salinity on freshwater ecosystems, the cosmopolitan toxic cyanobacterium Microcystis aeruginosa (Kützing) Kützing was selected as a model. It is known that this species that this species cannot proliferate in salinity >10-15 g∙L-1 but the limit of the adaptation to salinity remains to be investigated.Objectives To detect the maximum genetic adaptation capacity of three strains of M. aeruginosa (isolated from a freshwater reservoir with salinity <0.025 g∙L-1, i.e. without previous “evolution salinity history”) to the exposure of increased salinity.Methods An experimental evolutionary approach (ratchet experiment) was used. In order to analyze the differential evolutionary potential of three M. aeruginosa strains, two ratchet experiments were performed based on “soft” or “intense” exponentially increased salinity.Conclusions Acclimation (supported by the gene expression already present in the ancestral populations) was similar in the three strains in both ratchets experiments (“soft” and “intense”). A significant enhancement in resistance, supported by the selection of new genetic variants arising by mutations (genetic adaptation), was achieved in all derived populations in both ratchet experiments, and in the three strains. However, the results showed that the dynamics and the limit of genetic adaptation depend on previous adaptation history (historical contingency).
YR 2017
FD 2017-09-13
LK http://hdl.handle.net/10630/14464
UL http://hdl.handle.net/10630/14464
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.Spanish Ministry of Science and Innovation through CGL2014-53682-P project.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026