RT Conference Proceedings
T1 Regulation of Vitamin C content in higher plants
T2 Regulación del contenido en Vitamina C en plantas superiores
A1 Fenech-Torres, Mario
A1 Amorim-Silva, Vitor
A1 Smirnoff, Nicholas
A1 Botella-Mesa, Miguel Ángel
K1 Fotosíntesis
AB Ascorbic Acid (AsA, vitamin C) has multiple biological roles in plants. Although severalpathways for the novo synthesis of AsA have been reported, it is the L-Gal, also knownas the Smirnoff-Wheeler (SW), the main pathway operating in photosynthetic tissues inplants. Although there is abundant information of how these genes are regulated at thetranscriptional level, little is known about the regulation and the compartmentation of thispathway in higher plants. In order to investigate the localization of dynamics of proteinsinvolved in AsA biosynthesis in vivo at the cellular level, we have generated GFP fusionsusing the genomic region of the last five genes of the pathway, expected to be localizedin the cytoplasm. These constructs have been transformed in Arabidopsis thaliana (Col-0) plants and generated stable transgenic lines. Detailed localization and dynamics of theproteins are currently being investigated. In parallel, we have analyzed whether theseproteins form a complex in planta using co-immunoprecipitation assays using Nicotianabenthamiana. Our data indicate that most protein of the SW pathway show a nucleocytoplasmiclocalization and that they associate in vivo. Details of the results obtainedwill be presented.
YR 2017
FD 2017-06-23
LK http://hdl.handle.net/10630/13985
UL http://hdl.handle.net/10630/13985
LA eng
NO Es una comunicación a un Congreso Internacional de primeralínea en el campo objeto de la Tesis Doctoral de Mario Fenech
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. This research was supported by a grant from the Spanish Ministerio de Educación,Cultura y Deporte para la formación del Profesorado Universitario (FPU014/01974), aswell as by a project funded by the Spanish Ministerio de Ciencia e Innovación (BIO2014-55380R).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 1 mar 2026