RT Conference Proceedings T1 Vadose zone air as a biogenic source of methane in Nerja Cave system (South of Spain) A1 Vadillo-Pérez, Iñaki A1 Ojeda, Lucía A1 Etiope, Giuseppe A1 Benavente Herrera, José A1 Liñán-Baena, Cristina A1 Del Rosal Padial, Yolanda Remedios A1 Tapia-Paniagua, Silvana Teresa A1 Moriñigo-Gutiérrez, Miguel Ángel A1 Carrasco-Cantos, Francisco K1 Metano - Cueva de Nerja (Málaga) AB Subterranean air in karst cavities has often low methane contents in comparison to atmosphere and so karst systemshave been considered a sink of atmospheric methane. Methane-oxidizing bacteria have been hypothesized asresponsible for CH4 depletion in many caves around the world, although ionization radiation was also proposed aspossible mechanism for this process. We measured CO2 and CH4 concentration and their C isotopic composition(d13C-CO2 and d13C-CH4) over 2 years within the Nerja cave system (South Spain) and in 9 boreholes drilledinto the vadose zone (Triassic carbonate aquifer) surrounding the cave. According to d13C-CO2 and d13C-CH4vadose zone of this karst system is a source of biogenic methane, produced both by acetate fermentation and CO2reduction. Biogenic and atmospheric methane flows (along fractures from the vadose zone, and through ventilation,respectively) into the cave, where it is oxidized by methanotrophic bacteria that we detected on soil samplesinto the cave. PB EGU YR 2018 FD 2018 LK https://hdl.handle.net/10630/15590 UL https://hdl.handle.net/10630/15590 LA eng NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. DS RIUMA. Repositorio Institucional de la Universidad de Málaga RD 19 ene 2026