RT Conference Proceedings
T1 The potential of the RNAi technology, via SIGS, in the control of Botrytis cinerea in horticultural crops
A1 López-Laguna, Alba
A1 Vielba-Fernández, Alejandra
A1 Pérez-García, Alejandro
A1 Fernández-Ortuño, Dolores
K1 Microbiología agrícola
K1 Hongos en agricultura
AB Botrytis cinerea is one of the most important limiting factors for crop productionworldwide, as it demonstrated by the enormous annual intake of fungicides used for theircontrol to avoid crop losses. However, this fungus has been categorized by FRAC as a high-risk pathogen for fungicide resistance development. Another problem is related with the diversity of fungicidesavailable to growers, which according with the current European legislation on pesticides andthe European Green Deal, will be reduced by 50% by 2030. For all this, new low-impactsustainable solutions, obtained through new phytoprotection tools, to control B. cinerea areneeded. In this study, we intend to check if some emerging strategies such as RNA interferencetechnology (RNAi) could be valid sustainable solution and alternative to the use ofconventional chemical fungicides for the control of B. cinerea in crops of relevance. To achievethis goal, the SIGS (spray-induced gene silencing) approach, which concerns the exogenousapplication of double-stranded RNA (dsRNA), was tested. For it, ten double-stranded RNA(dsRNAs) were designed against the fungicide target’s genes and genes encoding proteins involved in virulence/pathogenicity of this fungus. The preliminary results obtained in in vitro tests have shown that the application of the different dsRNAs, individuallyand in combination, have significantly reduced the development of the fungus on differentculture media. In addition, this reduction was very promising on detached fruit and in plantaassays, demonstrating the potential of this technique in the control of B. cinerea. On the otherhand, the sustained release of the dsRNA-fungicides using nanoparticles as a carrier orstabilizer has also been analyzed.
YR 2022
FD 2022-06
LK https://hdl.handle.net/10630/25261
UL https://hdl.handle.net/10630/25261
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026