RT Journal Article
T1 Selection and induction of rat skeletal muscle-derived cells to the chondro-osteogenic lineage.
A1 Claros-Gil, Silvia
A1 Alonso, Mónica
A1 Becerra-Ratia, José
A1 Andrades-Gómez, José Antonio
K1 Células madre
K1 Huesos - Crecimiento - Modelos animales
AB Bone  marrow  (BM)  has  been  long  established  as  the  main  source  of  pluripotential  mesenchymal  stem  cells (MSCs),  and  has  been  so  far  the  main  recognized  source  of  osteoprogenitor  cells  that  contribute  to  the turnover  of  the skeletal  scaffold.  The  existence  of  an  osteoprogenitor  cell  in  other  connective  tissues  such  as  skeletal  muscle  has  been reported. In light of its availability and because of the relative ease of  muscle cell isolation, skeletal muscle is an attractive source of cells  for use in tissue engineering applications. The aim of this study  was to explore the potential to differentiate into  the  chondro-osteoblastic  lineage  of  a  plastic adhering  cell  population,  referred  t  as  skeletal  muscle-derived  cells (SMDCs), obtained from biopsies of rat skeletal muscle. SMDCs displayed a fibroblast-like morphology. Our study revealed that  the  isolated  cell  population  had  a  mesenchymal  origin  as  indicated  by  abundant  expression  of  STRO-1  and  CD166. Osteogenic markers like osteocalcin (OC), bone sialoprotein (BSP)and osteopontin (OP) gene expressions were detected by RT-PCR. When these cells were cultured in the presence of an osteo-inductive culture medium, positive staining for alkaline phosphatase  (ALP)  and  formation  of  mineralized  matrix  were  increased.  Furthermore  SMDCs  formed  bone  and  cartilage tissues in  vivo  when  placed  inside  of  diffusion  chambers  and  in  demineralized  bone  matrix  (DBM)  cylinders,  implanted subdermically  into  the  backs  of  rat  for  28  days.  In  conclusion,  this  experimental  procedure  is  capable  of  selecting  a  cell population obtained from the skeletal muscle that is able to complete the differentiation pathway leading to the formation of cartilage  and  bone.  In  this  respect  SMDCs  resemble  BM  stromal  cells  (BMSCs)  and  have  demonstrated  a  potential application for cartilage and bone tissue engineering.
PB CMB Association
YR 2008
FD 2008-10-26
LK https://hdl.handle.net/10630/32737
UL https://hdl.handle.net/10630/32737
LA eng
NO Política de acceso abierto tomada de: https://www.cellmolbiol.org/index.php/CMB/EditorialPolicies
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026