RT Journal Article
T1 Efficient DNA isolation from moroccan arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the rapd-pcr molecular technique
A1 Bakkali Yakhlefb, Salaheddine
A1 Guenoun, Imane
A1 kerdouh, Benaîssa
A1 Hamamouch, Noureddine
A1 Abourouh, Mohamed
A2 Departamento de Biología Vegetal (Botánica y Fisiología Vegetal), 
K1 Hojas (Botánica) - Análisis
K1 Botánica - Marruecos
AB Efficient DNA isolation from Moroccan Arar tree [Tetraclinis articulata (Vahl) Masters]leaves and optimization of the RAPD-PCR molecular technique. Molecular genetic analysis of Arar tree[Tetraclinis articulata (Vahl) Masters] is often limited by the availability of fresh tissue and an efficient andreliable protocol for high quality genomic DNA extraction. In this study, two DNA extraction protocols werespecifically developed for extracting high quality genomic DNA from Arar tree leaves: modified QIAgenDNA Kit and protocol developed by Ouenzar et al. (1998). DNA yield and purity were monitored by gelelectrophoresis and by determining absorbance at UV (A260/A280 and A260/A230). Both ratios were between 1.7and 2.0, indicating that the presence of contaminating metabolites was minimal. The DNA yield obtainedranged between 20 to 40 μg/g of plant materiel. The Ouenzar and collaborators protocol gave higher yieldbut was more time consuming compared to QIAgen Kit. However, both techniques gave DNA of goodquality that is amenable to RAPD-PCR reactions. Additionally, restriction digestion and PCR analyses of theobtained DNA showed its compatibility with downstream applications. Randomly Amplified PolymorphicDNA profiling from the isolated DNA was optimized to produce scorable and clear amplicons. The presentedprotocols allow easy and high quality DNA isolation for genetic diversity studies within Arar tree.
PB Universidad de Málaga. Servicio de Publicaciones
SN 02109506
YR 2010
FD 2010
LK http://hdl.handle.net/10630/4318
UL http://hdl.handle.net/10630/4318
LA eng
NO Acta Botánica Malacitana, Nº35, 2010, Págs. 97-106
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026