RT Conference Proceedings
T1 Adaptative mechanisms of cellulose synthesis under stress conditions
A1 Pagano Márquez, Raquel
A1 Macho, Alberto P.
A1 Castillo-Garriga, Araceli
A1 Amorim-Silva, Vitor
A1 Botella-Mesa, Miguel Ángel
K1 Celulosa
K1 Paredes celulares vegetales
AB Cellulose is the main structural compound of the plant cell wall and the most abundant biopolymer onEarth (Bar‐On et al 2018). The essential role of cellulose in plant development and defence highlights theimportance of understanding how its synthesis is regulated and will provide new tools to improve croptolerance to biotic and abiotic stresses.We identified that Tetratricopeptide Thioredoxin‐Like (TTL) proteins function as scaffold components ofbrassinosteroid signalling components (Amorim‐Silva et al 2019) and as new components of theCellulose Synthase Complex (CSC) and describe its unique dynamic association with the CSC undercellulose‐deficient conditions (Kesten, García‐Moreno, Amorim‐Silva et al 2022). The TTL‐CESAinteraction at the plasma membrane significantly increased under conditions that cause reducedcellulose content, such as salt stress and structural alterations of the CSCs. The relocalization of cytosolicTTLs to the active CSCs allows cellulose synthesis, mediated by a stress‐resilient cortical microtubulearray and the stabilization of the CSCs at the plasma membrane. TTLs carry this out by interacting withCellulose Synthase 1 and promoting the polymerization of microtubules, thus maintaining the stabilityand integrity of the complex. We propose that TTLs act as bridges connecting stress‐mediated cell wallmodification with the regulation of cellulose biosynthesis.We are currently investigating novel components involved in TTL function and how this protein family isregulated. Recently, we have identified the 14‐3‐3 proteins as interactors of TTL3. The 14‐3‐3s are afamily of proteins conserved in eukaryotes that target a wide number of proteins (Huang et al 2022). AnArabidopsis line overexpressing 14‐3‐3λ present phenotypes under stress consistent with defects incellulose biosynthesis. This study will elucidate a possible role of 14‐3‐3 proteins in TTL regulation andcellulose biosynthesis.
YR 2023
FD 2023-07-09
LK https://hdl.handle.net/10630/27386
UL https://hdl.handle.net/10630/27386
LA eng
NO This work was funded by the Spanish Ministry for Science and Innovation (MCIN/AEI/10.1 0 9/ 011000110 ) and the Andalusian Research Plan cofinanced by the EuropeanUnion(PAI I 2020-PY20_0008 and UMA20- E ERJA-02 ) to M.A.B.; V.A.-S. was supported by an Emerging Investigator research project (UMA20- E ERJA-007) and cofinanced by the“Programa Operativo E ER 201 -2020” and by the “Consejeria de Economia y Conocimiento de la Junta de Andalucia”. R.P.M. was supported by the Andalusian PRE OC_01fellowship. A.M. was supported by Shanghai Center for Plant Stress Biology.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 2 mar 2026