RT Journal Article
T1 Straightforward protocol for allele-specific chromatin conformation capture.
A1 Acemel, Rafael D.
A1 Tena, Juan J.
A1 Gómez-Skármeta, José Luis
A1 Fibla, Joan
A1 Royo-Sánchez-Palencia, José Luis
K1 Bioquímica - Métodos
K1 Cromosomas
K1 Cromatina
AB A key advance in our understanding of gene regulation came with the finding that the genomeundergoes three-dimensional nuclear folding in a genetically determined process. This 3Dconformation directly influences the association between enhancers and their target promoters. This complex interplay has been proven to be essential for gene regulation, and genetic variantsaffecting this process have been associated to human diseases. The development of newtechnologies that quantify these DNA interactions represented a revolution in the field. Highthroughput techniques like HiC provide a general picture of chromatin topology. However, theyoften lack resolution to evidence subtle effects that single nucleotide polymorphisms exert over the contacts between cis-regulatory regions and target promoters. Here we propose a costefficient approach to perform allele-specific chromatin conformation analysis. As a proof ofconcept, we analyzed the impact of a common deletion mapping between SIRPB1 promoter andone of its downstream enhancers
PB Elsevier
YR 2021
FD 2021-01-30
LK https://hdl.handle.net/10630/37428
UL https://hdl.handle.net/10630/37428
LA eng
NO Gene  2021 Jan 30;767:145185
NO https://openpolicyfinder.jisc.ac.uk/id/publication/16823
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026