RT Journal Article
T1 Pharmacological administration of the isoflavone daidzein enhances cell proliferation and reduces high fat diet-induced apoptosis and gliosis in the rat hippocampus.
A1 Rivera-González, Patricia
A1 Pérez-Martín, Margarita
A1 Pavón, Francisco Javier
A1 Serrano, Antonia
A1 Crespillo, Ana
A1 Cifuentes-Rueda, Manuel
A1 López-Ávalos, María  Dolores
A1 Mateos-Grondona, Jesús
A1 Vida, Margarita
A1 Fernández-Llebrez, Pedro
A1 Rodriguez-de-Fonseca, Fernando
A1 Suárez-Pérez, Juan
K1 Factores de transcripción
K1 Fitoestrógenos
K1 Estradiol
K1 Síndrome metabólico
K1 Obesidad
K1 Neurobiología del desarrollo
AB Soy extracts have been claimed to be neuroprotective against brain insults, an effect related to the estrogenic properties of isoflavones. However, the effects of individual isoflavones on obesity-induced disruption of adult neurogenesis have not yet been analyzed. In the present study we explore the effects of pharmacological administration of daidzein, a main soy isoflavone, in cell proliferation, cell apoptosis and gliosis in the adult hippocampus of animals exposed to a very high-fat diet. Rats made obese after 12-week exposure to a standard or high-fat (HFD, 60%) diets were treated with daidzein (50 mg kg(-1)) for 13 days. Then, plasma levels of metabolites and metabolic hormones, cell proliferation in the subgranular zone of the dentate gyrus (SGZ), and immunohistochemical markers of hippocampal cell apoptosis (caspase-3), gliosis (GFAP and Iba-1), food reward factor FosB and estrogen receptor alpha (ERα) were analyzed. Treatment with daidzein reduced food/caloric intake and body weight gain in obese rats. This was associated with glucose tolerance, low levels of HDL-cholesterol, insulin, adiponectin and testosterone, and high levels of leptin and 17β-estradiol. Daidzein increased the number of phospho-histone H3 and 5-bromo-2-deoxyuridine (BrdU)-ir cells detected in the SGZ of standard diet and HFD-fed rats. Daidzein reversed the HFD-associated enhanced immunohistochemical expression of caspase-3, FosB, GFAP, Iba-1 and ERα in the hippocampus, being more prominent in the dentate gyrus. These results suggest that pharmacological treatment with isoflavones regulates metabolic alterations associated with enhancement of cell proliferation and reduction of apoptosis and gliosis in response to high-fat diet.
PB PLOS
YR 2013
FD 2013-05-13
LK https://hdl.handle.net/10630/40137
UL https://hdl.handle.net/10630/40137
LA eng
NO Patricia Rivera, Margarita Pérez-Martín, Francisco J Pavón, Antonia Serrano, Ana Crespillo, Manuel Cifuentes, María-Dolores López-Ávalos, Jesús M Grondona, Margarita Vida, Pedro Fernández-Llebrez, Fernando Rodríguez de Fonseca, Juan Suárez. Pharmacological administration of the isoflavone daidzein enhances cell proliferation and reduces high fat diet-induced apoptosis and gliosis in the rat hippocampus. PLoS One . 2013 May 31;8(5):e64750. doi: 10.1371/journal.pone.0064750. Print 2013
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026